The Biotin Protein Labeling Kit provides a convenient way to label proteins with biotin. Biotin, SE (d - Biotin N - hydroxysuccinimide ester; Biotin, NHS ester) contains five-carbon spacer arm. The Biotin conjugated protein has less steric hindrance effect when it binds to streptavidin. The succinimidyl ester (SE) reactive form of Biotin reacts with the amine group on the protein and forms stable carboxamide bonds, which is identical to natural peptide bonds.
Biotin-protein conjugates are very stable and can withstand treatments during immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications without hydrolysis. The kit has all the essential components for performing the conjugation reaction and for purifying the conjugate.

This kit is optimized to conjugate Biotin, SE (d - Biotin N - hydroxysuccinimide ester; Biotin, NHS ester) to proteins (e.g., IgG).
It provides ample materials to perform three protein conjugations and purifications.
One conjugation reaction can label up to 200ug protein.
The entire process only takes about half an hour.