Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. Itbinds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a varietyof species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgGfrom guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG,but can also bind to the Fab region, making it useful for purification of F(ab‘) fragments of IgG. Due to it‘saffinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagentin biochemistry and immunology.
>98% by SDS-PAGE and HPLC analyses.
2、由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。
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