产品信息
荧光素标记
Unconjugated
抗原名称
CD49e (Integrin α5)
宿主
Mouse IgG1, κ
简单描述
Reacts with the α5 integrin, a dimer of 135/25 kDa, that associates with β1 integrin (CD29) to form VLA-5, a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, peripheral T cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone does not inhibit α5β1/ligand binding.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
Reacts with the α5 integrin, a dimer of 135/25 kDa, that associates with β1 integrin (CD29) to form VLA-5, a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, peripheral T cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone does not inhibit α5β1/ligand binding.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse IgG1, κ
克隆号
VC5
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing), Pig (Tested in Development)
背景
别名
Integrin α5 chain
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
2. Tran Van Nhieu G, Isberg RR. Bacterial internalization mediated by beta 1 chain integrins is determined by ligand affinity and receptor density. EMBO J. 1993; 12(5):1887-1895. (Biology).
3. Tran Van Nhieu G, Isberg RR. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. J Biol Chem. 1991; 266(36):24367-24375. (Biology).
参考图片
Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
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