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BD Pharmingen- PE Mouse Anti-Human CD79b_BD Pharmingen

艾美捷科技 • 2025年08月26日 15:27:48 • 抗体 • 阅读 30 • 标签
产品信息
荧光素标记
PE
抗原名称
CD79b
宿主
Mouse IgG1, κ
免疫原
Purified CD79αβ from Ramos B Cell Line
简单描述
Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Igα and Igβ, which have been designated in the Fifth International Leukocyte Workshop as CD79a and CD79b respectively. The CB3-1 monoclonal antibody specifically binds to CD79b, which is expressed on surface Ig (sIg)-positive lymphocytes and B-cell lines but only in the cytoplasm of sIg-negative cells including most terminal deoxynucleotidyl transferase (TdT) positive early pre-B and all cytoplasmic µ positive pre-B cell lines. Antibodies to CD79b are helpful in delineating signal transduction pathways activated via antibody receptors during different stages of B-cell differentiation.
商品描述
CB3-1 Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Igα and Igβ, which have been designated in the Fifth International Leukocyte Workshop as CD79a and CD79b respectively. The CB3-1 monoclonal antibody specifically binds to CD79b, which is expressed on surface Ig (sIg)-positive lymphocytes and B-cell lines but only in the cytoplasm of sIg-negative cells including most terminal deoxynucleotidyl transferase (TdT) positive early pre-B and all cytoplasmic µ positive pre-B cell lines. Antibodies to CD79b are helpful in delineating signal transduction pathways activated via antibody receptors during different stages of B-cell differentiation.
同种型
Mouse IgG1, κ
克隆号
克隆 CB3-1 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Tested During Development)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD79b
背景
别名
Ig-beta; IGB; B29
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Nakamura T, Kubagawa H, Cooper MD. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992; 89(18):8522-8526. (Biology). 2. Nakamura T, Sekar MC, Kubagawa H, Cooper MD. Signal transduction in human B cells initiated via Ig beta ligation.. Int Immunol. 1993; 5(10):1309-15. (Clone-specific). 3. Nakamura T. CD79 Workshop Panel Report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:180-182. 4. Sanchez M, Misulovin Z, Burkhardt AL. Signal transduction by immunoglobulin is mediated through Ig alpha and Ig beta. J Exp Med. 1993; 178(3):1049-1055. (Biology). 5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995. 6. Zabel BA, Nakae S, Zúñiga L, et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008; 205(10):2207-2220. (Immunogen).
数据库链接
Entrez-Gene ID
974

参考图片

Two-color flow cytometric analysis of CD79b expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD79b (Cat. No. 555679/561943) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 555899).The two color fluorescence dot plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Two-color flow cytometric analysis of CD79b expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD79b (Cat. No. 555679/561943) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 555899).The two color fluorescence dot plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

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