CD80 (B7-1)

Mouse IgM, κ

Reacts with a 60 kD transmembrane glycoprotein which was clustered as CD80 in the Fifth International Workshop on Human Differentiation Antigens. There have been several recent studies reporting discrepancies in the expression and function of CD80 when the BB1 clone is used compared to other anti-CD80 monoclonals (e.g., L307.4). Caution should therefore be used in the interpretation of data generated using BB1, as reports indicate that BB1 may recognize, in addition to CD80, CD74 and even possibly another as yet undefined molecule. CD80, a  member of the Ig supergene family, is expressed on activated B cells, macrophages, and dendritic cells. It is the ligand for two molecules expressed on T cells: CD28 and CD152 (CTLA-4). CD80 is also expressed in activated CD4+ and CD8+ T cells, appearing late after activation, suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway. The binding of CD28 molecule by CD28 mAb or by CD80 antigen results in T-cell activation and a signal for IL-2 production.

BB1 Reacts with a 60 kD transmembrane glycoprotein which was clustered as CD80 in the Fifth International Workshop on Human Differentiation Antigens. There have been several recent studies reporting discrepancies in the expression and function of CD80 when the BB1 clone is used compared to other anti-CD80 monoclonals (e.g., L307.4). Caution should therefore be used in the interpretation of data generated using BB1, as reports indicate that BB1 may recognize, in addition to CD80, CD74 and even possibly another as yet undefined molecule. CD80, a  member of the Ig supergene family, is expressed on activated B cells, macrophages, and dendritic cells. It is the ligand for two molecules expressed on T cells: CD28 and CD152 (CTLA-4). CD80 is also expressed in activated CD4+ and CD8+ T cells, appearing late after activation, suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway. The binding of CD28 molecule by CD28 mAb or by CD80 antigen results in T-cell activation and a signal for IL-2 production.

Mouse IgM, κ

克隆 BB1 (RUO)

Flow cytometry (Routinely Tested)

20 µl

Human (QC Testing)

CD80 (B7-1)

B7-1

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

研发参考(6) 1. Azuma M, Yssel H, Phillips JH, Spits H, Lanier LL. Functional expression of B7/BB1 on activated T lymphocytes. J Exp Med. 1993; 177(3):845-850. (Clone-specific). 2. Behrens L, Kerschensteiner M, Misgeld T, Goebels N, Wekerle H, Hohlfeld R. Human muscle cells express a functional costimulatory molecule distinct from B7.1 (CD80) and B7.2 (CD86) in vitro and in inflammatory lesions. J Immunol. 1998; 161(11):5943-5951. (Clone-specific). 3. Freeman GJ, Cardoso AA, Boussiotis VA, et al. The BB1 monoclonal antibody recognizes both cell surface CD74 (MHC class II-associated invariant chain) as well as B7-1 (CD80), resolving the question regarding a third CD28/CTLA-4 counterreceptor. J Immunol. 1998; 161(6):2708-2715. (Clone-specific). 4. Koulova L, Clark EA, Shu G, Dupont B. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. J Exp Med. 1991; 173(3):759-762. (Clone-specific). 5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995. 6. Schwartz RH. Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy. Cell. 1992; 71(7):1065-1068. (Clone-specific: Activation).