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BD Pharmingen- Biotin Mouse Anti-Human TCR _BD Pharmingen

艾美捷科技 • 2025年08月26日 15:47:18 • 抗体 • 阅读 57 • 标签
产品信息
抗原名称
TCR γδ
宿主
Mouse IgG1, κ
简单描述
The B1 monoclonal antibody specifically binds to the γδ T cell receptor (γδ TCR). This receptor complex consists of two disulfide-linked transmembrane glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). The γδ TCR is associated with the signal-transducing CD3 complex. The γδ TCR is expressed by thymocytes and by peripheral T cell subsets (γδ T cells) that are located in the blood, liver, skin and various lymphoid and mucosal tissues. γδ T cells contribute to both innate and adaptive immune responses to infections and tumors. Reports suggest that γδ T cells may also play roles in antigen presentation and the regulation of autoimmune responses.
商品描述
B1 The B1 monoclonal antibody specifically binds to the γδ T cell receptor (γδ TCR). This receptor complex consists of two disulfide-linked transmembrane glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). The γδ TCR is associated with the signal-transducing CD3 complex. The γδ TCR is expressed by thymocytes and by peripheral T cell subsets (γδ T cells) that are located in the blood, liver, skin and various lymphoid and mucosal tissues. γδ T cells contribute to both innate and adaptive immune responses to infections and tumors. Reports suggest that γδ T cells may also play roles in antigen presentation and the regulation of autoimmune responses.
同种型
Mouse IgG1, κ
克隆号
克隆 B1 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
TCR γδ
背景
别名
TCRgd; γδ TCR; TRG@, TRD@; TCRG, TCRD; TCR gamma delta
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(12) 1. Bai L, Picard D, Anderson B, et al. The majority of CD1d-sulfatide-specific T cells in human blood use a semiinvariant Vδ1 TCR.. Eur J Immunol. 2012; 42(9):2505-10. (Clone-specific). 2. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997. 3. Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). 4. Breit TM, Wolvers-Tettero IL, van Dongen JJ. Receptor diversity of human T-cell receptor gamma delta expressing cells. Prog Histochem Cytochem. 1992; 26(1-4):182-193. (Biology). 5. Davodeau F, Peyrat MA, Houde I, et al. Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science. 1993; 260(5115):1800-1802. (Clone-specific). 6. De Libero G. Sentinel function of broadly reactive human gamma delta T cells. Immunol Today. 1997; 18(1):22-26. (Biology). 7. Del Porto P, D'Amato M, Fiorillo MT, Tuosto L, Piccolella E, Sorrentino R. Identification of a novel HLA-B27 subtype by restriction analysis of a cytotoxic gamma delta T cell clone.. J Immunol. 1994; 153(7):3093-100. (Biology). 8. Fitzgerald KA, Callard RE. The cytokine factsbook., 2nd ed. / Katherine A. Fitzgerald ... [et al.].. San Diego: Academic Press; 2001:1-515. 9. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). 10. Kabelitz D, Pechhold K, Bender A, et al. Activation and activation-driven death of human gamma/delta T cells. Immunol Rev. 1991; 120:71-88. (Biology). 11. Kabelitz D. Function and specificity of human gamma/delta-positive T cells. Crit Rev Immunol. 1992; 11(5):281-303. (Biology). 12. Kabelitz D. γδ T-cells: cross-talk between innate and adaptive immunity. Cell Mol Life Sci. 2011; 68(14):2331-2333. (Biology).

参考图片

Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody (Cat. No. 561807/561806/555332/555916) and Biotin Mouse Anti-Human TCR γδ antibody (Cat No. 555716). Secondary staining was carried out with PE Streptavidin (Cat. No. 554061), and erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric plot showing the correlated expression of TCR γδ versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACScan™.

Two-color flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody (Cat. No. 561807/561806/555332/555916) and Biotin Mouse Anti-Human TCR γδ antibody (Cat No. 555716). Secondary staining was carried out with PE Streptavidin (Cat. No. 554061), and erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color flow cytometric plot showing the correlated expression of TCR γδ versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACScan™.

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