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BD Pharmingen- PE Mouse Anti-Human Ig - Light Chain_BD Pharmingen

艾美捷科技 • 2025年08月26日 16:35:35 • 抗体 • 阅读 38 • 标签
产品信息
荧光素标记
PE
宿主
Mouse IgG1, κ
简单描述
The G20-193 monoclonal antibody specifically binds to human immunoglobulin light chain, kappa (κ). It does not react with human immunoglobulin λ light chains or human immunoglobulin heavy chains.
商品描述
G20-193 The G20-193 monoclonal antibody specifically binds to human immunoglobulin light chain, kappa (κ). It does not react with human immunoglobulin λ light chains or human immunoglobulin heavy chains.
同种型
Mouse IgG1, κ
克隆号
克隆 G20-193 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
Ig, κ Light Chain
背景
别名
Ig, kappa Light Chain; IGKC; HCAK1; Km; Ig kappa chain C region
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Cary SP, Lee J, Wagenknecht R, Silverman GJ. Characterization of superantigen-induced clonal deletion with a novel clan III-restricted avian monoclonal antibody: exploiting evolutionary distance to create antibodies specific for a conserved VH region surface. J Immunol. 2000; 164(9):4730-4741. (Clone-specific: Flow cytometry). 2. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). 3. Widhopf GF, 2nd, Brinson DC, Kipps TJ, Tighe H. Transgenic expression of a human polyreactive Ig expressed in chronic lymphocytic leukemia generates memory-type B cells that respond to nonspecific immune activation. J Immunol. 2004; 172(4):2092-2099. (Biology: Flow cytometry).
数据库链接
Entrez-Gene ID
3514

参考图片

Multicolor flow cytometric analysis of Ig κ Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; Left Panel) or PE Mouse Anti-Human Ig κ Light Chain antibody (Cat. No. 555792/562052; Right Panel). The bivariate pseudocolor density plots showing the correlated expression of Ig κ Light Chain (or Ig isotype control staining) versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD™ LSR II Flow Cytometer System and FloJo™ software.

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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