BD Via-Probe™ is a convenient, ready-to-use solution of the nucleic acid dye, 7-Amino-actinomycin D (7-AAD) that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between the 7-AAD, PE and FITC fluorescence emissions. BD Via-Probe™ fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).  This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3. This reagent does not require dilution, use 20 µL/test (1x10^6 cells) and incubate for 10 minutes before analysis.

(RUO)

Flow cytometry (Tested During Development)

7-AAD

Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium azide.

Development References(2) 1. O'Brien MC, Bolton WE. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry. 1995; 19(3):243-255. (Biology). 2. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry. 1992; 13(2):204-208. (Biology).