BD Pharmingen- AKP Mouse Anti-Human IgE_BD Pharmingen
产品信息
抗原名称
IgE
宿主
Mouse IgG2a, κ
简单描述
The G7-26 monoclonal antibody specifically recognizes the constant region of human Immunoglobulin E (IgE). It does not crossreact with other immunoglobulin heavy chain isotypes. IgE exists in a transmembrane form that is expressed by B lymphocytes and serves as an antigen receptor. Soluble IgE is produced and secreted by activated B cells and plasma cells. IgE may bind through its constant region to cell surface receptors, such as the high-affinity (FcεRI) or low-affinity (FcεRII/CD23) receptors for IgE. FcεRI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes. FcεRII/CD23 is expressed by B cells and by some other cell types including T cells, monocytes, eosinophils, neutrophils, follicular dendritic cells, and Langerhans cells. Crosslinking of Fc receptor-bound IgE antibodies by multivalent antigens or allergens can induce phagocytosis or the cellular release of inflammatory mediators. Although IgE can provide immune protection against pathogenic parasites, it may also play a central role in a variety of allergic disorders.
商品描述
G7-26
The G7-26 monoclonal antibody specifically recognizes the constant region of human Immunoglobulin E (IgE). It does not crossreact with other immunoglobulin heavy chain isotypes. IgE exists in a transmembrane form that is expressed by B lymphocytes and serves as an antigen receptor. Soluble IgE is produced and secreted by activated B cells and plasma cells. IgE may bind through its constant region to cell surface receptors, such as the high-affinity (FcεRI) or low-affinity (FcεRII/CD23) receptors for IgE. FcεRI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes. FcεRII/CD23 is expressed by B cells and by some other cell types including T cells, monocytes, eosinophils, neutrophils, follicular dendritic cells, and Langerhans cells. Crosslinking of Fc receptor-bound IgE antibodies by multivalent antigens or allergens can induce phagocytosis or the cellular release of inflammatory mediators. Although IgE can provide immune protection against pathogenic parasites, it may also play a central role in a variety of allergic disorders.
同种型
Mouse IgG2a, κ
克隆号
克隆 G7-26 (RUO)
产品详情
AKP
Alkaline phosphatase (AKP) is an enzyme that is capable of dephosphorylating various substrates which generates a measurable colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with AKP and used in applications such as i) ELISA that employs a plate reader to measure absorbance of the colored product or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
应用
实验应用
ELISA (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
IgE
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Brinkley M. A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjug Chem. 1992; 3(1):2-13. (Biology).
2. Mattson G, Conklin E, Desai S, Nielander G, Savage MD, Morgensen S. A practical approach to crosslinking. Mol Biol Rep. 1993; 17(3):167-183. (Biology).
3. Weston PD, Devries JA, Wrigglesworth R. Conjugation of enzymes to immunoglobulins using dimaleimides. Biochim Biophys Acta. 1980; 612(1):40-90. (Biology).
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