Genebridege公司的FLPe表达质粒707-710可以促成大肠杆菌中以FLP为媒介的定点重组，在30°C时以低拷贝数传播。Flpe基因由λR启动子驱动，受控于不耐热cI857阻遏蛋白。温度从30°C上升至37°C会引发FLPe重组酶暂态表达。由于pSC101复制原点的缘故，表达质粒在37°C时不再复制，因此它们zui终会消失。与野生型FLP蛋白相比，FLPe拥有更好的耐热性，在37 – 40°C时显示出更强的重组酶活性产品信息如下：

All Red/ET Recombination Kits come with tailored control experiments and harbor the same Red/ET proficiency plasmid.

 

 Quick & Easy BAC Modification Kit 

For all types of BAC modifications.
ID: K001	690.00 €*	Details	Manual	Order

 

 Counter Selection BAC Modification Kit 

For advanced BAC modifications using a counter-selection cassette, also for bacterial chromosomes.
ID: K002	795.00 €*	Details	Manual	Order

 

 BAC Subcloning Kit 

For subcloning of DNA fragments from BACs and cosmids.
ID: K003	620.00 €*	Details	Manual	Order

 

 Quick & Easy Conditional Knock Out Kit - FRT 

For fast and simple integration of FRT-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）.
ID: K004	690.00 €*	Details	Manual	Order

 

 Quick & Easy Conditional Knock Out Kit - loxP 

For fast and simple integration of loxP-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）.
ID: K005	690.00 €*	Details	Manual	Order

 

 Quick & Easy E.coli Gene Deletion Kit 

For fast knockouts or modifications of genes on the E.coli chromosome.
ID: K006	620.00 €*	Details	Manual	Order

Red/ET Recombination Strains

 

 E. coli strain GB05-dir 

Direct cloning-proficient E. coli strain for linear plus linear homologous recombination
ID: K008	590.00 €*	Details	Manual	Order

 

 E. coli strain GB08-red 

Recombineering-proficient E. coli strain for linear plus circular homologous recombination
ID: K009	590.00 €*	Details	Manual	Order

Selection Cassettes

Also available are accessory selection cassettes designed for use in Eukaryotes or Prokaryotes, some flanked by loxP- or FRT-sites. These sites will allow the subsequent removal of the selection marker by a Cre- or FLP-recombinase step if required. These cassettes may be very helpful for generating targeting constructs in mammals, or for introducing sequences without leaving a selection marker behind.

 

 PGK-gb2-neo 

Pro- and Eukaryotic Neomycin Selection Cassette
ID: A001	290.00 €*	Details	Manual	Order

 

 FRT-PGK-gb2-neo-FRT 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites
ID: A002	290.00 €*	Details	Manual	Order

 

 loxP-PGK-gb2-neo-loxP 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by loxP-sites
ID: A003	290.00 €*	Details	Manual	Order

 

 FRT-PGK-gb2-neo-FRT-loxP 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional downstream loxP site
ID: A004	290.00 €*	Details	Manual	Order

 

 loxP-FRT-PGK-gb2-neo-FRT 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional upstream loxP site
ID: A005	290.00 €*	Details	Manual	Order

 

 FRT-gb2-cm-FRT 

Prokaryotic Chloramphenicol Selection Cassette flanked by FRT-sites
ID: A006	290.00 €*	Details	Manual	Order

 

 loxP-gb2-cm-loxP 

Prokaryotic Chloramphenicol Selection Cassette flanked by loxP-sites
ID: A007	290.00 €*	Details	Manual	Order

 

 FRT-gb2-amp-FRT 

Prokaryotic Ampicillin Selection Cassette flanked by FRT-sites
ID: A008	290.00 €*	Details	Manual	Order

 

 loxP-gb2-amp-loxP 

Prokaryotic Ampicillin Selection Cassette flanked by loxP-sites
ID: A009	290.00 €*	Details	Manual	Order

 

 FRT-PGK-gb2-hygro-FRT 

FRT flanked, Pro- and Eukaryotic Hygromycin Selection Cassette
ID: A010	290.00 €*	Details	Manual	Order

 

 loxP-PGK-gb2-hygro-loxP 

loxP flanked, Pro- and Eukaryotic Hygromycin Selection Cassette
ID: A011	290.00 €*	Details	Manual	Order

 

 iCre-FRT-neo-FRT 

Codon improved Cre （iCre） with attached FRT flanked, Pro- and Eukaryotic Neomycin Selection Cassette
ID: A012	325.00 €*	Details	Manual	Order

 

Expression Plasmids

The following recombinase expression plasmids are very useful to excise FRT- or loxP-flanked DNA stretches （such as selection markers） during a modification task. The plasmids are easily transformed into cells resulting in an activation of the recombinases in vivo.

 

 

 707-FLPe, tet 

Flp Expression Plasmid with tetracycline resistance marker for use in E.coli only
ID: A104	290.00 €*	Details	Manual	Order

 

 

 708-FLPe, cm 

Flp Expression Plasmid with chloramphenicol resistance marker for use in E.coli only
ID: A105	290.00 €*	Details	Manual	Order

 

 

 709-FLPe, ap 

Flp Expression Plasmid with ampicillin resistance marker for use in E.coli only
ID: A106	275.00 €*	Details	Manual	Order

 

 

 710-FLPe, km 

Flp Expression Plasmid with kanamycin resistance marker for use in E.coli only
ID: A107	275.00 €*	Details	Manual	Order

 

 

 705-Cre;cm 

Cre Expression Plasmid with chloramphenicol resistance marker for use in E.coli only
ID: A112	290.00 €*	Details	Manual	Order

 

 

 706-Cre;tet 

Cre Expression Plasmid with tetracycline resistance marker for use in E.coli only
ID: A113	290.00 €*	Details	Manual	Order

 

 

 pCAGGS-FLPe 

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Academia）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement.
ID: A201	375.00 €*	Details	Manual	Order

 

 

 pCAGGS-FLPe 

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Industry）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement.
ID: A202	3250.00 €*	Details	Manual	Order

 

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