原核表达载体指:能携带插入的外源核酸序列进入原核细胞中进行复制的载体。
编辑本段3. 获得含重组表达质粒的表达菌种
(1) 将连接产物转化大肠杆菌DH5α,根据重组载体的标志(抗Amp或蓝白斑)作筛选,挑取单斑,碱裂解法小量抽提质粒,双酶切初步鉴定。
(2)测序验证目的基因的插入方向及阅读框架均正确,进入下步操作。否则应筛选更多克隆,重复亚克隆或亚克隆至不同酶切位点。
(3) 以此重组质粒DNA转化表达宿主菌的感受态细胞。
| 1. pET系列载体 | ||
| 1. pET系列载体 | ||
| 78-pet-5a | pET-5a | 2ug |
| 78-pet11a | pET11a | 2ug |
| 78-pet-11b | pET-11b | 2ug |
| 78-pet-11c | pET-11c | 2ug |
| 78-pet-15b | pET-15b | 2ug |
| 78-pet-16b(+) | pET-16b(+) | 2ug |
| 78-pet17b | pET17b | 2ug |
| 78-pet-19b | pET-19b | 2ug |
| 78-pet-20b(+) | pET-20b(+) | 2ug |
| 78-pet21a(+) | pET21a(+) | 2ug |
| 78-pet-21b | pET-21b | 2ug |
| 78-pet-21d(+) | pET-21d(+) | 2ug |
| 78-pet-22b | pET-22b | 2ug |
| 78-pet-23a(+) | pET-23a(+) | 2ug |
| 78-pet-24 | pET-24 | 2ug |
| 78-pet24a(+) | pET24a(+) | 2ug |
| 78-pet24-b | pET24-b | 2ug |
| 78-pet-25b(+) | pET-25b(+) | 2ug |
| 78-pet26b | pET26b | 2ug |
| 78-pet27-b | pET27-b | 2ug |
| 78-pet-28a | pET-28a | 2ug |
| 78-pet-28b | pET-28b | 2ug |
| 78-pet-28c | pET-28c | 2ug |
| 78-pet29a | pET29a | 2ug |
| 78-pet-29b | pET-29b | 2ug |
| 78-pet-30a | pET-30a | 2ug |
| 78-pet-30b | pET-30b | 2ug |
| 78-pet-30c | pET-30c | 2ug |
| 78-pet-32a | pET-32a | 2ug |
| 78-pet32(+)c | pET32(+)c | 2ug |
| 78-pet-32b | PET-32b | 2ug |
| 78-pet35b(+) | pET35b(+) | 2ug |
| 78-pet38b(+) | pET38b(+) | 2ug |
| 78-pet-39b | pET-39b | 2ug |
| 78-pet-3a | pET-3a | 2ug |
| 78-pet-40b(+) | pET-40b(+) | 2ug |
| 78-pet-41a | pET-41a | 2ug |
| 78-pet-42a(+) | pET-42a(+) | 2ug |
| 78-pet-42b | pET-42b | 2ug |
| 78-pet-43a | pET-43a | 2ug |
| 78-pet-43b | pET-43b | 2ug |
| 78-pet-49b(+) | pET-49b(+) | 2ug |
| 78-PET-31b(+) | ET31b(+) | 2ug |
| 78-pet-41b(+) | pET-41b(+) | 2ug |
| 78-pet41Ek-Lic | pET41Ek-Lic | 2ug |
| 2. PTYB 系列载体 | 2. PTYB 系列载体 | |
| 78-TYB1 | TYB1 | 2ug |
| 78-TYB2 | TYB2 | 2ug |
| 78-TYB11 | TYB11 | 2ug |
| 78-TYB12 | TYB12 | 2ug |
| 3. pGEX系列载体 | 3. pGEX系列载体 | |
| 78-pGEX-4T-1 | pGEX-4T-1 | 2ug |
| 78-pGEX-4T-2 | pGEX-4T-2 | 2ug |
| 78-pGEX-6P-1 | pGEX-6P-1 | 2ug |
| 78-pGEX-20T | pGEX-20T | 2ug |
| 78-pGEX-4T-3 | pGEX-4T-3 | 2ug |
| 78-pGEX-6P-2 | pGEX-6P-2 | 2ug |
| 78-pGEX-5X-1 | pGEX-5X-1 | 2ug |
| 78-pGEX-2TK | pGEX-2TK | 2ug |
| 78-pGEX | pGEX | 2ug |
| 78-pGEX-KG | pGEX-KG | 2ug |
| 4. pBAD系列载体 | 4. pBAD系列载体 | |
| 78-pBAD24 | pBAD24 | 2ug |
| 78-pBAD43 | pBAD43 | 2ug |
| 78-pBADHis A | pBADHis A | 2ug |
| 78-pBADHisB | pBADHisB | 2ug |
| 78-pBADHisC | pBADHisC | 2ug |
| 5.原核融合表达载体 | 5.原核融合表达载体 | |
| 78-PProEX-HTa | pProEX-HTa | 2ug |
| 78-PProEX-HTb | pProEX-HTb | 2ug |
| 78-PProEX-HTc | pProEX-HTc | 2ug |
| 78-PMAL-c4x | pMAL-c4x | 2ug |
| 78-PEYFP-mem | pEYFP-mem | 2ug |
| 78-PEXP1-DEST | pEXP1-DEST | 2ug |
| 6. 其他载体 | ||
| 78-Pbv220 | pbv220 | 2ug |
| 78-PET-DsbA | pET-DsbA | 2ug |
| 78-PET-GST | pET-GST | 2ug |
| 78-PET-His | pET-His | 2ug |
| 78-KD3 | KD3 | 2ug |
| 78-PLLP-Om78-PA | pLLP-OmpA | 2ug |
| 78-PLLP-STII | pLLP-STII | 2ug |
| 78-PMAL-C2X | pMAL-C2X | 2ug |
| 78-PMAL-P2X | pMAL-P2X | 2ug |
| 78-PMBP-P | pMBP-P | 2ug |
| 78-PQE30 | pQE30 | 2ug |
| 78-PQE31 | pQE31 | 2ug |
| 78-PQE-32 | pQE-32 | 2ug |
| 78-PRSET C | pRSET C | 2ug |
| 78-PRSET-A/B | pRSET-A/B | 2ug |
| 78-PTH-304-18Z | pTH-304-18Z | 2ug |
| 78-PTrcHis B | pTrcHis B | 2ug |
| 78-PTrcHis C | pTrcHis C | 2ug |
| 78-PTrcHisA | pTrcHisA | 2ug |
| 78-PTrxFus | pTrxFus | 2ug |
| 78-PTWIN1 | pTWIN1 | 2ug |
| 78-PG-KJE8 | pG-KJE8 | 2ug |
| 78-PGro7 | pGro7 | 2ug |
| 78-PKJE7 | pKJE7 | 2ug |
| 78-PG-Tf2 | pG-Tf2 | 2ug |
| 78-PTf16 | pTf16 | 2ug |
| 78-PQE40 | pQE40 | 2ug |
| 78-PCMV-myc | pCMV-myc | 2ug |
| 78-PQE80 | pQE80 | 2ug |
| 78-PSE380 | pSE380 | 2ug |
| 78-PQE-9 | pQE-9 | 2ug |
| 78-PQE-60 | pQE-60 | 2ug |
| 78-PQE-70 | pQE-70 | 2ug |
| 78-PTWIN2 | pTWIN2 | 2ug |
| 78-PCFS119 | PCFS119 | 2ug |
| 78-PVSV-G | pVSV-G | 2ug |
| 78-PCDFDuet-1 | pCDFDuet-1 | 2ug |
| 78-PSecTag2 HygroA | pSecTag2 HygroA | 2ug |
本文来自投稿,不代表本人立场,如若转载,请注明出处:http://www.iamyjet.com/kangti/2298.html