High transfection efficiency and significantly lower toxicity levels for a wide range of cell lines
Significantly improved transfection performance in a number of primary and hard-to-transfect cell lines
Optimized protocols for over 30 different cell lines are available so much less time is needed for evaluation and optimization
FreeStyle™ MAX Transfection Reagent
Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells
Used for a large-scale transient protein production with milligrams of protein yield
Lipofectamine™ RNAiMAX Transfection Reagent
Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects
Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
Compatibility with a broad range of cell types providing the most versatile approach to all of your gene silencing experiments
Lipofectamine™ 2000 Transfection Reagent
Achieves highest efficiency and expression results for plasmid or RNAi transfections
Works effectively with many cell types (adherent or suspension)
Easy and fast protocol – no need for wash steps before or after transfection
Complexes can be added to cells growing in serum-containing media
Ideal if transfecting at confluencies of 90% or greater (minimizes cytotoxicity following transfection)
Lipofectamine™ 2000 CD Transfection Reagent
Same performance as Lipofectamine™ 2000, certified animal-origin free (“CD” = chemically defined)
Oligofectamine™ Transfection Reagent
Transfection of antisense oligonucleotides
293fectin™ Transfection Reagent
Used for transient protein production in combination with the FreeStyle™ 293 Expression System
Optimized for suspension FreeStyle™ 293-F cells
Cellfectin® Transfection Reagent
Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells
DMRIE-C Transfection Reagent
Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.
Lipofectamine™ Transfection Reagent
First generation reagent for plasmid DNA transfections
In most cases, Lipofectamine™ 2000 provides better performance than Lipofectamine™ Transfection Reagent
Lipofectin® Transfection Reagent
First generation reagent for plasmid DNA transfections
In most cases, Lipofectamine™ 2000 provides better performance than Lipofectin® Transfection Reagent
Optifect™ Transfection Reagent
Broad use reagent designed for low confluency applications (<70% confluent at the time of transfection)
Useful for cell lines that are sensitive to transfection reagents
Plasmid DNA Transfection
To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents to mediate efficient delivery into the cell's nucleus. This process is essential for subsequent protein expression of the gene of interest. Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism of adding a DNA Plasmid into a mammalian cell is known as plasmid transfection.
Invitrogen offers a wide range of transfection reagents for your specific needs. For cell types specific protocols, please refer to Transfection Selection Tool.
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Plasmid Transfection Reagents
Electroporation Instruments & Reagents
Featured Product - Lipofectamine™ LTX with PLUS™ Reagent
Our latest and most efficient Lipofectamine™ product. The best choice for transfecting plasmid DNA into standard and other difficult to transfect cell types; highest transfection efficiency and viability with superb expression for all cell types.
High transfection efficiency and high gene expression with minimal cytotoxicity in a broad range of cells
Significantly improved transfection performance in a number of primary and disease-relevant cell types
No washes or media changes required - just set up and go!
In 90% of the cell lines that we have tested, transfection performance is enhanced by using Lipofectamine™ LTX and PLUS™ Reagent together.
Learn more about Lipofectamine™ LTX with PLUS™ Reagent