Merck蛋白抽提
MERCK蛋白抽提
1、BugBuster®蛋白抽提试剂——尤其适用于GST融合蛋白的抽提BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。 2、BugBuster Master Mix预混蛋白抽提试剂BugBuster Master Mix是将BugBuster蛋白抽提试剂，Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一种非常方便使用的蛋白抽提试剂，有助于在*化条件下获得可溶活性蛋白。| Product | Size | Cat.No. | Price（￥） |
| BugBuster® Protein Extraction Reagent | 100 ml | 70584-3 | 750.00 |
| 500 ml | 70584-4 | 3,789.00 | |
| BugBuster® Master Mix | 100 ml | 71456-3 | 1,700.00 |
| 500 ml | 71456-4 | 5,200.00 | |
| CytoBuster™ Protein Extraction Reagent | 50 ml | 71009-3 | 824.00 |
| 250 ml | 71009-4 | 3,122.00 |
| | Comparison of E. coli lysis methods 50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity. |
- ² 蛋白抽提操作中降低样品粘度
- ² 双向电泳中样品制备过程
- ² 重组蛋白样品制备中去除核酸污染
| Product | Size | Cat.No. | Price（￥） |
| Benzonase® Nuclease, Purity > 90% | 10 ku | 70746-3 | 1,200.00 |
| 2.5 ku | 70746-4 | 400.00 |
| | Viscosity reduction by Benzonase Nuclease E. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at |
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