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BD Pharmingen- FITC Rat Anti-Mouse Ig- - Light Chain_BD Pharmingen

艾美捷科技 • 2025年08月19日 13:37:44 • 抗体 • 阅读 43 • 标签
产品信息
荧光素标记
FITC
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
免疫原
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
简单描述
The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
187.1 The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
克隆号
克隆 187.1 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry, Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
Ig, κ Light Chain
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1) 1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen).
数据库链接
Entrez-Gene ID
16071

参考图片

Detection of intracellular kappa light chains in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described in the recommended assay procedure below using FITC-conjugated 187.1 mAb (solid line, Cat. No. 550003) or the matched isotype control, FITC-conjugated R3-34 mAb (dotted line, Cat. No. 554684). Flow cytometry was performed on a BD FACSCalibur ™ flow cytometry system.

Detection of intracellular kappa light chains in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described in the recommended assay procedure below using FITC-conjugated 187.1 mAb (solid line, Cat. No. 550003) or the matched isotype control, FITC-conjugated R3-34 mAb (dotted line, Cat. No. 554684). Flow cytometry was performed on a BD FACSCalibur ™ flow cytometry system.

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