BD Pharmingen- FITC Hamster IgG2 - Isotype Control_BD Pharmingen
产品信息
荧光素标记
FITC
免疫原
Keyhole Limpet Hemocyanin
简单描述
The B81-3 monoclonal antibody was raised against keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells or cell lines. Intended for use as a hamster immunoglobulin isotype control, the B81-3 antibody can be used as a negative control to help differentiate non-specific background signal when compared against a signal from a specific hamster antibody. The purified B81-3 antibody may also serve as a useful standard for some ELISA applications. KLH is a oxygen carrying metalloprotein from a species of keyhole limpet that lives off the coast of California and is often used as a carrier protein to help elicit immune responses to haptens. Since KLH is phylogenetically distant from many mammalian proteins, it often has very minimal to no cross-reactivity for many immunological assays.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
B81-3
The B81-3 monoclonal antibody was raised against keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells or cell lines. Intended for use as a hamster immunoglobulin isotype control, the B81-3 antibody can be used as a negative control to help differentiate non-specific background signal when compared against a signal from a specific hamster antibody. The purified B81-3 antibody may also serve as a useful standard for some ELISA applications. KLH is a oxygen carrying metalloprotein from a species of keyhole limpet that lives off the coast of California and is often used as a carrier protein to help elicit immune responses to haptens. Since KLH is phylogenetically distant from many mammalian proteins, it often has very minimal to no cross-reactivity for many immunological assays.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Armenian Hamster IgG2, κ
克隆号
克隆 B81-3 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
目标/特异性
KLH
背景
别名
Anti-KLH
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen).
参考图片
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8594.html