Granulocyte macrophage colony stimulating factor (GM-CSF) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Human GM-CSF is a 14 - 17 kD multiple glycosylated protein containing 123 amino acid residues.  Recombinant human GM-CSF (Cat. No. 550068) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing bovine serum albumin with no preservatives.  Recombinant human GM-CSF is  ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human GM-CSF, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Human (QC Testing)

GM-CSF

Frozen aqueous buffered solution containing BSA.

Development References(4) 1. Gasson JC. Molecular physiology of granulocyte-macrophage colony-stimulating factor. Blood. 1991; 77(6):1131-1145. (Biology). 2. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Biology). 3. Miyatake S, Otsuka T, Yokota T, Lee F, Arai K. Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes. EMBO J. 1985; 4(10):2561-2568. (Biology). 4. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).