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BD Pharmingen- Purified Mouse Anti-Human CD1d_BD Pharmingen

艾美捷科技 • 2025年08月19日 13:57:52 • 抗体 • 阅读 18 • 标签
产品信息
抗原名称
CD1d
宿主
Mouse BALB/c IgG1, κ
免疫原
Human CD1d Recombinant Protein
简单描述
The CD1d42 monoclonal antibody recognizes CD1d. Cell surface CD1d is structurally homologous to Class I MHC molecules. It consists of a glycosylated type I transmembrane α chain (43-49 kDa) that is non-covalently associated with β2-microglobulin. CD1d is a member of the CD1 family of molecules, which belong to the immunoglobulin superfamily. Sequence homology data classifies the CD1 molecules into two groups. Group 1 includes CD1a, CD1b and CD1c molecules; group 2 includes CD1d molecules and their homologs in other species. CD1d is expressed on cortical thymocytes, B cells, dendritic cells, monocytes, and some nonlymphoid cells including intestinal epithelial cells, hepatocytes and keratinocytes. It is not expressed on resting mature T cells. Studies suggest that CD1d participates in lipid antigen presentation to CD1d-restricted NKT cells.
商品描述
CD1d42 The CD1d42 monoclonal antibody recognizes CD1d. Cell surface CD1d is structurally homologous to Class I MHC molecules. It consists of a glycosylated type I transmembrane α chain (43-49 kDa) that is non-covalently associated with β2-microglobulin. CD1d is a member of the CD1 family of molecules, which belong to the immunoglobulin superfamily. Sequence homology data classifies the CD1 molecules into two groups. Group 1 includes CD1a, CD1b and CD1c molecules; group 2 includes CD1d molecules and their homologs in other species. CD1d is expressed on cortical thymocytes, B cells, dendritic cells, monocytes, and some nonlymphoid cells including intestinal epithelial cells, hepatocytes and keratinocytes. It is not expressed on resting mature T cells. Studies suggest that CD1d participates in lipid antigen presentation to CD1d-restricted NKT cells.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 CD1d42 (also known as 42.1) (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD1d
背景
别名
R3; R3G1; HMC class I antigen-like glycoprotein CD1D
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4) 1. Exley M, Garcia J, Wilson SB, et al. CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. Immunology. 2000; 100(1):37-47. (Biology). 2. Hong S, Scherer DC, Singh N. Lipid antigen presentation in the immune system: lessons learned from CD1d knockout mice. Immunol Rev. 1999; 169:31-44. (Biology). 3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997. 4. Somnay-Wadgaonkar K, Nusrat A, Kim HS. Immunolocalization of CD1d in human intestinal epithelial cells and identification of a beta2-microglobulin-associated form. 1999; 11(3):383-392. (Biology).

参考图片

Flow cytometric analysis of peripheral blood lymphocytes. Whole blood was stained with Purified Mouse Anti-Human CD1d (Cat. No. 550254; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No.555746, dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of peripheral blood lymphocytes. Whole blood was stained with Purified Mouse Anti-Human CD1d (Cat. No. 550254; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No.555746, dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

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