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BD Pharmingen- FITC Mouse Anti-Human CD103_BD Pharmingen

艾美捷科技 • 2025年08月19日 14:01:03 • 抗体 • 阅读 13 • 标签
产品信息
荧光素标记
FITC
抗原名称
CD103 (Integrin aE)
宿主
Mouse BALB/c IgG1, κ
免疫原
Human MAPS16 T Cell Line
简单描述
The Ber-ACT8 monoclonal antibody specifically binds to the human mucosal lymphocyte antigen 1 (HML-1). This is a 175 kDa type I transmembrane glycoprotein, also known as integrin αE (ITGAE, Integrin alpha-E) and CD103. It is found on >90% of intestinal intraepithelial lymphocytes (iIEL) associated with integrin β7. CD103 is also expressed on lamina propria T lymphocytes in the intestine and on phytohemagglutinin-stimulated peripheral blood lymphocytes. It is rarely expressed on resting peripheral blood lymphocytes. It has been suggested that CD103 may have an accessory function for activation of iIEL. CD103 has been reported as a useful tool in hairy cell leukemia research.
商品描述
Ber-ACT8 The Ber-ACT8 monoclonal antibody specifically binds to the human mucosal lymphocyte antigen 1 (HML-1). This is a 175 kDa type I transmembrane glycoprotein, also known as integrin αE (ITGAE, Integrin alpha-E) and CD103. It is found on >90% of intestinal intraepithelial lymphocytes (iIEL) associated with integrin β7. CD103 is also expressed on lamina propria T lymphocytes in the intestine and on phytohemagglutinin-stimulated peripheral blood lymphocytes. It is rarely expressed on resting peripheral blood lymphocytes. It has been suggested that CD103 may have an accessory function for activation of iIEL. CD103 has been reported as a useful tool in hairy cell leukemia research.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 Ber-ACT8 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD103 (Integrin αE)
背景
别名
ITGAE; Integrin alpha-E; ITAE; HML-1 antigen; Mucosal lymphocyte 1; HUMINAE
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

参考图片

Flow cytometric analysis of CD103 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated (3 days) peripheral blood mononuclear cells were stained with either FITC Mouse IgG1, k Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-Human CD103 antibody (Cat. No. 550259; solid line histogram). The fluorescence histograms showing CD103 expression (or Ig Isotype control) were derived from events with the forward and side light-scatter characteristics of stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Profile of PHA-stimulated (3 days) peripheral blood mononuclear cells (PBMC) analyzed by flow cytometry

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