BD Pharmingen- Purified Hamster Anti-Mouse CD11c_BD Pharmingen
产品信息
抗原名称
CD11c (Integrin aX)
宿主
Armenian Hamster IgG1, λ2
免疫原
C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
简单描述
The HL3 monoclonal antibody specifically binds to the integrin αx chain of gp150, 95 (CD11c/CD18). CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells. It is upregulated on IEL and lymph-node T cells following
in vivo
activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b.
商品描述
HL3
The HL3 monoclonal antibody specifically binds to the integrin αx chain of gp150, 95 (CD11c/CD18). CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells. It is upregulated on IEL and lymph-node T cells following
in vivo
activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b.
同种型
Armenian Hamster IgG1, λ2
克隆号
克隆 HL3 (RUO)
浓度
125 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD11c (Integrin αX)
背景
别名
Cd11c; Itgax; Integrin alpha-X; Integrin αX; Cr4; Complement receptor 4
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Burt BM, Plitas G, Stableford JA, et al. CD11c identifies a subset of murine liver natural killer cells that responds to adenoviral hepatitis. J Leukoc Biol. 2008; 84(4):1039-1046. (Clone-specific).
2. Fagarasan S, Muramatsu M, Suzuki K, Nagaoka H, Hiai H, Honjo T. Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora. Science. 2002; 298(5597):1424-1427. (Clone-specific: Immunofluorescence).
3. Huleatt JW, Lefrançois L. Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo.. J Immunol. 1995; 154(11):5684-93. (Immunogen: Immunoprecipitation).
4. Larson RS, Springer TA. Structure and function of leukocyte integrins. Immunol Rev. 1990; 114:181-217. (Biology).
5. Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990; 171(5):1753-1771. (Biology).
6. Pulendran B, Lingappa J, Kennedy MK, et al. Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice. J Immunol. 1997; 159(5):2222-2231. (Clone-specific: Immunohistochemistry).
参考图片
Expression of CD11c on spleen dendritic cells. The frozen section of normal mouse spleen was stained with Purified Hamster Anti-Mouse CD11c (Cat. No. 550283/553799) and three-step staining was carried out with the Anti-Hamster Ig HRP detection kit (Cat. No. 551012). Note the brown labeling of irregular-shaped cells in the red pulp surrounding lymphoid follicles.
Expression of CD11c on spleen dendritic cells. The frozen section of normal mouse spleen was stained with Purified Hamster Anti-Mouse CD11c (Cat. No. 550283/553799) and three-step staining was carried out with the Anti-Hamster Ig HRP detection kit (Cat. No. 551012). Note the brown labeling of irregular-shaped cells in the red pulp surrounding lymphoid follicles.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8621.html