BD Pharmingen- Purified Rat Anti-Mouse CD62P_BD Pharmingen
产品信息
抗原名称
CD62P (P-Selectin)
宿主
Rat LEW, also known as Lewis IgG1, λ
免疫原
P-selectin-IgG1 Fusion
简单描述
The RB40.34 monoclonal antibody specifically binds to mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.
商品描述
RB40.34
The RB40.34 monoclonal antibody specifically binds to mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.
同种型
Rat LEW, also known as Lewis IgG1, λ
克隆号
克隆 RB40.34 (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, ELISA, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-paraffin (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD62P (P-Selectin)
背景
别名
P-selectin; Selp; LECAM3; LYAM3; PADGEM; GMP-140; Grmp
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Aigner S, Ruppert M, Hubbe M, et al. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol. 1995; 7(10):1557-1565. (Biology).
2. Bosse R, Vestweber D. Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum. Eur J Immunol. 1994; 24(12):3019-3024. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation).
3. Ley K, Bullard DC, Arbones ML, et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med. 1995; 181(2):669-675. (Clone-specific: Blocking).
4. Yang J, Galipeau J, Kozak CA, Furie BC, Furie B. Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. Blood. 1996; 87(10):4176-4186. (Biology).
参考图片
Immunohistochemical staining of megakaryocytes and platelets. The frozen section of normal mouse spleen was reacted with RB40.34 mAb. Megakaryocytes and platelets are identified by the intense brown staining.
Immunohistochemical staining of megakaryocytes and platelets. The frozen section of normal mouse spleen was reacted with RB40.34 mAb. Megakaryocytes and platelets are identified by the intense brown staining.
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