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BD Pharmingen- PE Mouse anti-human CD142_BD Pharmingen

艾美捷科技 • 2025年08月19日 14:26:48 • 抗体 • 阅读 29 • 标签
产品信息
荧光素标记
PE
抗原名称
CD142
宿主
Mouse IgG1, κ
免疫原
Human Tissue Factor Protein
简单描述
The HTF-1 monoclonal antibody specifically binds to CD142. CD142 is a 45-47 kDa, single chain, type I transmembrane glycoprotein also known as Tissue Factor (TF). CD142 has been referred in the literature as coagulation Factor III or thromboplastin and it is expressed on activated endothelial cells and lipopolysaccharide (LPS)-stimulated monocytes/macrophages. TF associates with factor VIIa to form a complex and acts as an enzyme that initiates the blood coagulation cascade. It is known as the major initiator of clotting in normal hemostasis. CD142 can be induced by various inflammatory mediators in monocytes and vascular endothelial cells. This antibody is useful in inflammation, thrombosis and hemostasis research.
商品描述
HTF-1 The HTF-1 monoclonal antibody specifically binds to CD142. CD142 is a 45-47 kDa, single chain, type I transmembrane glycoprotein also known as Tissue Factor (TF). CD142 has been referred in the literature as coagulation Factor III or thromboplastin and it is expressed on activated endothelial cells and lipopolysaccharide (LPS)-stimulated monocytes/macrophages. TF associates with factor VIIa to form a complex and acts as an enzyme that initiates the blood coagulation cascade. It is known as the major initiator of clotting in normal hemostasis. CD142 can be induced by various inflammatory mediators in monocytes and vascular endothelial cells. This antibody is useful in inflammation, thrombosis and hemostasis research.
同种型
Mouse IgG1, κ
克隆号
克隆 HTF-1 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD142 (Tissue Factor)
背景
别名
Tissue factor; TF; TFA; Coagulation factor III; F3; Thromboplastin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

参考图片

LPS-stimulated peripheral blood monocytes analyzed by flow cytometry

LPS-stimulated peripheral blood monocytes analyzed by flow cytometry

Flow cytometric analysis of CD142 expression on activated human monocytes. Human peripheral blood mononuclear cells (PBMC) were stimulated (4 hr) with 0.1 µg/mL lipopolysaccharide (LPS; Sigma-Aldrich, Cat. No. L-2654). The cells were harvested, washed and pre-blocked for 10 min with 5% human AB serum. The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human CD142 antibody (Cat. No. 550312/561713; solid line histogram). The fluorescence histograms showing CD142 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact activated monocytes. Flow cytometric analysis and data analysis was performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.

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