This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (5000 pg/mL), then adding 100 µL of the 5000 pg/mL standard to both that well and the 2500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 2500 pg/mL standard to the 1250 pg/mL well. Continue these dilutions to the 78 pg/mL standard well, out of which the extra 100 µL should be discarded.