BD Pharmingen- Purified Mouse Anti-Human CD3_BD Pharmingen
产品信息
抗原名称
CD3
宿主
Mouse IgG2a, κ
简单描述
The HIT3a monoclonal antibody specifically binds to the human CD3ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).
商品描述
HIT3a
The HIT3a monoclonal antibody specifically binds to the human CD3ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).
同种型
Mouse IgG2a, κ
克隆号
克隆 HIT3a (RUO)
浓度
125 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
反应种属
Human (QC Testing)
目标/特异性
CD3
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Beverley PC, Callard RE. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981; 11(4):329-334. (Biology).
2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
3. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
参考图片
Immunohistochemical staining of T lymphocytes. Frozen sections of normal human tonsil was reacted with the CD3 antibody. T lymphocytes can be identified by the intense brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of T lymphocytes. Frozen sections of normal human tonsil was reacted with the CD3 antibody. T lymphocytes can be identified by the intense brown labeling of their cell membranes. Amplification 20X.
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