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BD Pharmingen- Purified Mouse Anti-Itk_BD Pharmingen

艾美捷科技 • 2025年08月19日 15:12:16 • 抗体 • 阅读 12 • 标签
产品信息
抗原名称
ITK
宿主
Mouse IgG1
免疫原
Fusion protein with aa.1-26 of human/mouse Itk
简单描述
Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
商品描述
2F12 Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
同种型
Mouse IgG1
克隆号
克隆 2F12 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), In vitro kinase assay (Reported)
反应种属
Human (QC Testing), Mouse (Tested in Development)
目标/特异性
Itk
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.

参考图片

Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.

Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, Cat. No. 550503) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Itk is identified as a band at ~72 kDa.

Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.

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