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BD Pharmingen- Purified Mouse Anti-Human CD120a_BD Pharmingen

艾美捷科技 • 2025年08月19日 15:14:06 • 抗体 • 阅读 13 • 标签
产品信息
抗原名称
CD120a
宿主
Mouse BALB/c IgG2a, κ
免疫原
Human CD120a Recombinant Protein
简单描述
The TNFR1-B1 monoclonal antibody specifically recognizes the extracellular domain of CD120a. CD120a is encoded by TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A). CD120a is also known as the 55 kDa receptor for the human cytokines, tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-a). This receptor is referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI). TNFRI are expressed by a variety of cell lines, tumor cells, and normal cell types including T cells, monocytes, macrophages, neutrophils, endothelial cells, hepatocytes, chondrocytes, and fibroblasts. Naive B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes and platelets are uniformly negative for TNFRI expression. MABTNFR1-B1 specifically binds to natural and recombinant truncated forms of TNFRI.
商品描述
MABTNFR1-B1 The TNFR1-B1 monoclonal antibody specifically recognizes the extracellular domain of CD120a. CD120a is encoded by TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A). CD120a is also known as the 55 kDa receptor for the human cytokines, tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-a). This receptor is referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI). TNFRI are expressed by a variety of cell lines, tumor cells, and normal cell types including T cells, monocytes, macrophages, neutrophils, endothelial cells, hepatocytes, chondrocytes, and fibroblasts. Naive B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes and platelets are uniformly negative for TNFRI expression. MABTNFR1-B1 specifically binds to natural and recombinant truncated forms of TNFRI.
同种型
Mouse BALB/c IgG2a, κ
克隆号
克隆 MABTNFR1-B1 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD120a (TNF Receptor 1A)
背景
别名
TNF Receptor Type I; TNFR1; TNFR55; TNFR60; TNFRSF1A
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.

参考图片

Flow cytometric analysis of CD120a expression of cell surface TNFRI by non-activated human PBMC or RBC. Human PBMC (left panel) and red blood cells (right panel) isolated by density centrifugation (Ficoll-Paque™) were stained with Purified Mouse Anti-Human CD120a (Cat No. 550514) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption), (Cat No. 553999) and PE Streptavidin (Cat. No. 554061) in a three-step staining protocol to amplify immunofluorescent signals. Staining with the Purified Mouse Anti-Human CD120a (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in the left panel are gated on the lymphocyte population. Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

Flow cytometric analysis of CD120a expression of cell surface TNFRI by non-activated human PBMC or RBC. Human PBMC (left panel) and red blood cells (right panel) isolated by density centrifugation (Ficoll-Paque™) were stained with Purified Mouse Anti-Human CD120a (Cat No. 550514) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption), (Cat No. 553999) and PE Streptavidin (Cat. No. 554061) in a three-step staining protocol to amplify immunofluorescent signals. Staining with the Purified Mouse Anti-Human CD120a (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in the left panel are gated on the lymphocyte population. Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

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