BD Retrievagen A (pH 6.0) is an antigen retrieval system that is recommended for Zinc-fixed and Formalin-fixed paraffin embedded tissue sections that show sub-optimal or no reactivity with antibodies for immunohistochemical staining. 10% neutral buffered formalin fixes the tissue by cross-linking proteins that may cause a conformational change or "masking" of the antigenic epitope leading to reduced or absent antibody reactivity. The BD Retrievagen A (pH 6.0) is designed to unmask the epitope and cause the enhancement of antibody staining. The proposed mechanism by which most of the antigen retrieval systems work is through breaking the cross-linking bonds formed between the protein molecules. It is important to realize that in spite of antigen retrieval, some antibodies do not stain on formalin-fixed paraffin embedded sections. In these situations fixing the tissue in a less harsh fixative like the IHC zinc fixative developed by BD Pharmingen (Cat. No. 550523) may be helpful. In many cases our zinc-fixative preserves the epitope leading to good antibody staining at the same time depicting good tissue morphology. However, there are a few antigens that are not preserved by any gentle fixation, and in these situations use of fresh frozen tissue for immunohistochemical staining is the only alternative.

(RUO)

Retrievagen A (pH6.0)

研发参考(3) 1. Chan JK. Advances in immunohistochemical techniques: toward making things simpler, cheaper, more sensitive, and more reproducible. Adv Anat Pathol. 1998; 5(5):314-325. (Biology). 2. Pileri SA, Roncador G, Ceccarelli C, et al. Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J Pathol. 1997; 183(1):116-123. (Biology). 3. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991; 39(6):741-748. (Biology).