BD Pharmingen- Purified Rat Anti-Mouse CD45_BD Pharmingen
产品信息
抗原名称
CD45
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
免疫原
Mouse Thymus / Spleen
简单描述
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
商品描述
30-F11
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
克隆号
克隆 30-F11 (RUO)
浓度
62.5 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
CD45
背景
别名
Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
2. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90. (Immunogen).
3. van Ewijk W, van Soest PL, van den Engh GJ. Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200. J Immunol. 1981; 127(6):2594-2604. (Biology).
参考图片
Immunohistochemical staining of CD45. Zinc-fixed paraffin-embedded sections of normal mouse spleen were stained with Purified Rat Anti-Mouse CD45 (Cat. No. 550539). CD45 positive cells can be identified by the brown labeling of the cell membranes.
Immunohistochemical staining of CD45. Zinc-fixed paraffin-embedded sections of normal mouse spleen were stained with Purified Rat Anti-Mouse CD45 (Cat. No. 550539). CD45 positive cells can be identified by the brown labeling of the cell membranes.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8710.html