BD Pharmingen- Biotin Mouse Anti-Mouse I-A-d_BD Pharmingen
产品信息
抗原名称
I-Ad
宿主
Mouse SJL IgG2b, κ
免疫原
BALB/c mouse splenocytes
简单描述
The AMS-32.1 antibody specifically reacts with the I-A[d] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.
商品描述
AMS-32.1
The AMS-32.1 antibody specifically reacts with the I-A[d] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.
同种型
Mouse SJL IgG2b, κ
克隆号
克隆 AMS-32.1 (RUO)
浓度
62.5 µg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
I-A[d]
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen: Flow cytometry).
2. Matsumoto S, Setoyama H, Umesaki Y. Differential induction of major histocompatibility complex molecules on mouse intestine by bacterial colonization. Gastroenterology. 1992; 103(6):1777-1782. (Clone-specific: Immunohistochemistry).
3. Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific).
参考图片
Immunohistochemistry of I-A[d] positive cells. Frozen sections of mouse thymus were reacted with the anti-I-A[d] antibody. Cells expressing the MHC class II molecule I-A[d] can be identified by the intense brown labeling of their cell membranes. Magnification 20x.
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