产品信息
荧光素标记
PE
抗原名称
CD62P (P-Selectin)
宿主
Mouse BALB/c IgG1, κ
免疫原
Activated Human Platelets
简单描述
The AC1.2 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin may mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.
商品描述
The AC1.2 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin may mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.
同种型
Mouse BALB/c IgG1, κ
克隆号
AC1.2
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)
背景
别名
SELP; PSEL; P-selectin; PADGEM; LECAM3; GMP-140; GRMP; GMP140
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(13)
1. Albelda SM. Role of integrins and other cell adhesion molecules in tumor progression and metastasis. Lab Invest. 1993; 68(1):4-17. (Biology).
2. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology).
3. Dore M, Korthuis RJ, Granger DN, Entman ML, Smith CW. P-selectin mediates spontaneous leukocyte rolling in vivo. Blood. 1993; 82(4):1308-1316. (Biology).
4. Groves RW, Allen MH, Ross EL, Ahsan G, Barker JN, MacDonald DM. Expression of selectin ligands by cutaneous squamous cell carcinoma. Am J Pathol. 1993; 143(4):1220-1225. (Biology).
5. Johnson-Tidey RR, McGregor JL, Taylor PR, Poston RN. Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 1994; 144(5):952-961. (Biology).
6. Kansas GS. Selectins and their ligands: current concepts and controversies. Blood. 1997; 88(9):3259-3287. (Biology).
7. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
8. Larsen E, Celi A, Gilbert GE, et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.. Cell. 1989; 59(2):305-12. (Immunogen).
9. Lorant DE, Topham MK, Whatley RE, et al. Inflammatory roles of P-selectin. J Clin Invest. 1993; 92(2):559-570. (Biology).
10. Nagata K, Tsuji T, Todoroki N, et al. Activated platelets induce superoxide anion release by monocytes and neutrophils through P-selectin (CD62). J Immunol. 1993; 151(6):3267-3273. (Biology).
11. Pigott R, Power C. The Adhesion Molecule Facts Book. 1993:173.
12. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
13. Stone JP, Wagner DD. P-selectin mediates adhesion of platelets to neuroblastoma and small cell lung cancer. J Clin Invest. 1993; 92(2):804-813. (Biology).
数据库链接
Entrez-Gene ID
6403
参考图片
Flow cytometric analysis of CD62P expression on activated Cynomologus macaque (Macaca fascicularis) platelets. Platelets were stained with either PE Mouse Anti-Human CD62P (Cat. No. 550561; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable platelets.
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