BD Pharmingen- Purified Mouse Anti-Rat CD45_BD Pharmingen
产品信息
抗原名称
CD45
宿主
Mouse BALB/c IgG1, κ
免疫原
Leukocyte Common Antigen-enriched Glycoprotein Fraction from Wistar Rat Thymocytes
简单描述
The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family; its intracellular (COOH-terminal) region contains two PTP catalytic domains while the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), and differing levels of glycosylation. The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
商品描述
OX-1
The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family; its intracellular (COOH-terminal) region contains two PTP catalytic domains while the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), and differing levels of glycosylation. The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 OX-1 (RUO)
浓度
250 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
反应种属
Rat (QC Testing)
目标/特异性
CD45
背景
别名
Ptprc; Lca; Leucocyte common antigen; RT7; T200
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
2. Kampinga J, Aspinall R. Thymocyte differentiation and thymic micro-environment development in the fetal rat thymus: a immunohistological approach. In: Kendall MD, Ritter MA, ed. Role of the Thymus in Tolerance Induction, Vol 3. New York: Harwood Academic Publishers; 1990:149-186.
3. Sunderland CA, McMaster WR, Williams AF. Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. Eur J Immunol. 1979; 9(2):155-159. (Immunogen: Flow cytometry).
4. Woollett GR, Barclay AN, et al. Molecular and antigenic heterogeneity of the rat leukocyte common antigen from thymocytes and T and B lymphocytes. . Eur J Immunol. 1985; 15:168-173. (Biology).
参考图片
Immunohistochemical staining of rat leukocytes: Zinc-fixed paraffin sections of normal rat intestine were reacted with the anti-CD45 antibody. Leukocytes expressing CD45 can be identified by the brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of rat leukocytes: Zinc-fixed paraffin sections of normal rat intestine were reacted with the anti-CD45 antibody. Leukocytes expressing CD45 can be identified by the brown labeling of their cell membranes. Amplification 20X.
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