BD Pharmingen- Purified Mouse Anti-Ki-67_BD Pharmingen
产品信息
抗原名称
Ki-67
宿主
Mouse IgG1, κ
免疫原
Human Ki-67
简单描述
Recognizes Ki-67, a nuclear cell proliferation-associated antigen expressed in all active stages of the cell cycle. Ki-67 is revealed as a double band (345 and 395 kD) in western blot analysis of proliferating cells. B56 was developed using an immunogen composed of the immunodominant epitope of the Ki-67 protein. Antibodies B56 and MIB 1 react with this immunogen. Flow cytometric analysis reveals that the binding of B56-PE can be blocked by MIB 1 purified antibody. Immunohistochemistry analysis demonstrates a staining pattern similar to MIB 1 on both frozen and paraffin-embedded tissue sections.
商品描述
B56
Recognizes Ki-67, a nuclear cell proliferation-associated antigen expressed in all active stages of the cell cycle. Ki-67 is revealed as a double band (345 and 395 kD) in western blot analysis of proliferating cells. B56 was developed using an immunogen composed of the immunodominant epitope of the Ki-67 protein. Antibodies B56 and MIB 1 react with this immunogen. Flow cytometric analysis reveals that the binding of B56-PE can be blocked by MIB 1 purified antibody. Immunohistochemistry analysis demonstrates a staining pattern similar to MIB 1 on both frozen and paraffin-embedded tissue sections.
同种型
Mouse IgG1, κ
克隆号
克隆 B56 (RUO)
浓度
250 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing), Mouse (Tested in Development), Rat,Rhesus (Reported)
目标/特异性
Ki-67
背景
别名
MKI67; Antigen identified by monoclonal antibody Ki-67; KIA
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
参考图片
Immunohistochemistry of Ki-67 positive cells. Formalin-fixed paraffin embedded sections of normal human tonsil were reacted with the anti-Ki-67 antibody. Proliferating cells expressing Ki-67 can be identifed by the intense brown labeling of their cell nuclei. Magnification 20X.
Immunohistochemistry of Ki-67 positive cells. Formalin-fixed paraffin embedded sections of normal human tonsil were reacted with the anti-Ki-67 antibody. Proliferating cells expressing Ki-67 can be identifed by the intense brown labeling of their cell nuclei. Magnification 20X.
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