产品信息
荧光素标记
PE
抗原名称
CD34
宿主
Mouse IgG1, κ
简单描述
The 563 monoclonal antibody specifically binds to CD34. CD34 is a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 563 belongs to the class III epitope; it is resistant to neuraminidase, chymopapain and glycoprotease. This antibody is able to block the binding of other CD34 mAbs like 581.
Clone 563 reacts with the human form of the 105-120 kDa heavily O-glycosylated and N-glycosylated transmembrane glycoprotein, CD34, expressed on stem/progenitor hematopoietic cells, endothelial cells and some tissue. Clone 563 also cross reacts with a subset of peripheral blood mononuclear cells of both rhesus and cynomolgus macaque monkeys, but not baboon. The distribution of stem-like cells is similar to that observed with peripheral blood mononuclear cells from normal human donors.
商品描述
563
The 563 monoclonal antibody specifically binds to CD34. CD34 is a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 563 belongs to the class III epitope; it is resistant to neuraminidase, chymopapain and glycoprotease. This antibody is able to block the binding of other CD34 mAbs like 581.
Clone 563 reacts with the human form of the 105-120 kDa heavily O-glycosylated and N-glycosylated transmembrane glycoprotein, CD34, expressed on stem/progenitor hematopoietic cells, endothelial cells and some tissue. Clone 563 also cross reacts with a subset of peripheral blood mononuclear cells of both rhesus and cynomolgus macaque monkeys, but not baboon. The distribution of stem-like cells is similar to that observed with peripheral blood mononuclear cells from normal human donors.
同种型
Mouse IgG1, κ
克隆号
克隆 563 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Rhesus, Cynomolgus (QC Testing), Human (Tested in Development)
目标/特异性
CD34
背景
别名
gp 105-120
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Egeland T, Tjonnfjord G, Steen R, Gaudernack G, Thorsby E. Positive selection of bone marrow-derived CD34 positive cells for possible stem cell transplantation. Transplant Proc. 1993; 25(1):1261-1263. (Biology).
2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
4. Nishio H, Tada J, Hashiyama N, Hirn J, Ingles-Esteven J, Suda T. CD34. 1999. Available: http://mpr.nci.nih.gov/prow/guide/968267813_g.htm 2006, February 8.
5. Owens MA, Loken MR. Peripheral blood stem cell quantitation. In: Owens MA, Loken MR. Flow Cytometry Principles for Clinical Laboratory Practice. New York: John Wiley & Sons; 1995:128.
6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
数据库链接
Entrez-Gene ID
947
参考图片
Profile of CD34b Ab reactivity on peripheral blood (CD14-negative) stem cells from rhesus macaque (Macaca mulatta) analyzed by flow cytometry
Flow cytometry analysis of CD34 expression on Rhesus macaque (Macaca mulatta) stem cells (CD14-negative) from peripheral blood mononuclear cells. Rhesus PBMCs were stained with V450 Mouse Anti-Human CD14 (Cat. No. 561390) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; data not shown) or PE Mouse Anti-Human CD34 (Cat. No. 550619/550761). Fluorescent dot plot depicting CD34 expression was derived from gated events with the forward and side light-scatter characteristics of viable, CD14-negative cells.
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