BD Pharmingen- PE Mouse Anti-Human CD83_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD83
宿主
Mouse IgG1, κ
免疫原
Human CD83 transfected COS cells
简单描述
The HB15e monoclonal antibody specifically binds to a 45 kDa type 1 transmembrane glycoprotein member of the Ig superfamily. CD83 is composed of a single V-type Ig extracellular domain with a C-terminal cytoplasmic tail. Cell surface CD83 is expressed mainly by follicular dendritic cells, circulating dendritic cells, interdigitating dendritic cells in lymphoid tissues, in vitro-generated dendritic cells and thymic dendritic cells. However, its expression is not restricted to dendritic cells. CD83 is also expressed on some germinal center B cells and some lymphoblastoid cell lines. Although its function is not known, it may play a role in cell-cell interaction during antigen presentation.
Clone HB15e also cross reacts with a subset of in vitro-generated dendritic-like cells of both rhesus and cynomolgus macaque monkeys. Non-human primate peripheral blood mononuclear cells (PBMC) were cultured in presence of human GM-CSF, human IL-4 and human TNFα for 5-10 days. The distribution of these in vitro-generated dendritic-like cells is similiar to that observed with in vitro-generated dendritic cells from normal human donors.
商品描述
HB15e
The HB15e monoclonal antibody specifically binds to a 45 kDa type 1 transmembrane glycoprotein member of the Ig superfamily. CD83 is composed of a single V-type Ig extracellular domain with a C-terminal cytoplasmic tail. Cell surface CD83 is expressed mainly by follicular dendritic cells, circulating dendritic cells, interdigitating dendritic cells in lymphoid tissues, in vitro-generated dendritic cells and thymic dendritic cells. However, its expression is not restricted to dendritic cells. CD83 is also expressed on some germinal center B cells and some lymphoblastoid cell lines. Although its function is not known, it may play a role in cell-cell interaction during antigen presentation.
Clone HB15e also cross reacts with a subset of in vitro-generated dendritic-like cells of both rhesus and cynomolgus macaque monkeys. Non-human primate peripheral blood mononuclear cells (PBMC) were cultured in presence of human GM-CSF, human IL-4 and human TNFα for 5-10 days. The distribution of these in vitro-generated dendritic-like cells is similiar to that observed with in vitro-generated dendritic cells from normal human donors.
同种型
Mouse IgG1, κ
克隆号
克隆 HB15e (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)
目标/特异性
CD83
背景
别名
BL11; HB15; B-cell activation protein
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Hart DN. Dendritic cells: unique leukocyte populations which control the primary immune response. Blood. 1997; 90(9):3245-3287. (Biology).
2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
5. Summers KL, Daniel PB, O'Donnell JL, Hart DN. Dendritic cells in synovial fluid of chronic inflammatory arthritis lack CD80 surface expression. Clin Exp Immunol. 1995; 100(1):81-89. (Biology).
6. Weissman D, Li Y, Ananworanich J, et al. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1. Proc Natl Acad Sci U S A. 1995; 92(3):826-830. (Biology).
7. Zhou LJ, Schwarting R, Smith HM, Tedder TF. A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily. J Immunol. 1992; 149(2):735-742. (Biology).
8. Zhou LJ, Tedder TF. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J Immunol. 1995; 154(8):3821-3835. (Biology).
数据库链接
Entrez-Gene ID
9308
参考图片
Flow cytometric analysis of CD83 expression on cultured Rhesus macaque (Macaca mulatta) dendritic-like cells. Cultured dendritic-like cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram) or PE Mouse Anti-Human CD83 (Cat. No. 556855/561959/550634; solid line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering events of viable cells.
Flow cytometric analysis of CD83 expression on cultured Rhesus macaque (Macaca mulatta) dendritic-like cells. Cultured dendritic-like cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram) or PE Mouse Anti-Human CD83 (Cat. No. 556855/561959/550634; solid line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering events of viable cells.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8746.html