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BD Pharmingen- FITC Mouse Anti-Human CD63_BD Pharmingen

艾美捷科技 • 2025年08月19日 16:12:41 • 抗体 • 阅读 8 • 标签
产品信息
荧光素标记
FITC
抗原名称
CD63
宿主
Mouse BALB/c IgG1, κ
免疫原
Human Splenic Adherent Cells
简单描述
The H5C6 monoclonal antibody specifically binds to CD63. CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). It is widely expressed on surface and in the cytoplasm of various hematopoietic (monocytes, macrophages) and non-hematopoietic (endothelium, fibroblasts, osteoclasts, smooth muscle) cells. CD63 plays roles in mediating cellular adhesion and motility.
商品描述
H5C6 The H5C6 monoclonal antibody specifically binds to CD63. CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). It is widely expressed on surface and in the cytoplasm of various hematopoietic (monocytes, macrophages) and non-hematopoietic (endothelium, fibroblasts, osteoclasts, smooth muscle) cells. CD63 plays roles in mediating cellular adhesion and motility.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 H5C6 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
CD63
背景
别名
LAMP-3; ME491; MLA-1; Granulophysin; Ptgr40; NGA; gp55
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
967

参考图片

Flow cytometric analysis of CD63 expression on Rhesus macaque peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin (Sigma-Aldrich, Cat. No.T8885), and then fixed with 2% formaldehyde. After washing, the fixed platelets were stained with either FITC Mouse Anti-Human CD63 antibody (Cat. No. 557288/550759/561924; solid line histogram) or with FITC Mouse IgG1 , κ Isotype Control (Cat. No. 555748; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets.

Flow cytometric analysis of CD63 expression on Rhesus macaque peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin (Sigma-Aldrich, Cat. No.T8885), and then fixed with 2% formaldehyde. After washing, the fixed platelets were stained with either FITC Mouse Anti-Human CD63 antibody (Cat. No. 557288/550759/561924; solid line histogram) or with FITC Mouse IgG1 , κ Isotype Control (Cat. No.  555748; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets.

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