BD Pharmingen- Purified Mouse Anti-Human p16_BD Pharmingen
产品信息
抗原名称
CDKN2A (p16-INK4a)
宿主
Mouse IgG1
免疫原
Human p16 Recombinant Protein
简单描述
Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16. The gene, now also known as the CDKN2 gene, has been found to be mutated in a very high percentage of tumors, including 75% of melanoma cell lines.
商品描述
G175-405
Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16. The gene, now also known as the CDKN2 gene, has been found to be mutated in a very high percentage of tumors, including 75% of melanoma cell lines.
同种型
Mouse IgG1
克隆号
克隆 G175-405 (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
CDKN2A (p16-INK4a)
背景
别名
p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Kamb A, Gruis NA, Weaver-Feldhaus J, et al. A cell cycle regulator potentially involved in genesis of many tumor types. Science. 1994; 264(5157):436-440. (Biology).
2. Marx J. Link to hereditary melanoma brightens mood for p16 gene. Science. 1994; 265(5177):1364-1365. (Biology).
3. Serrano M, Hannon GJ, Beach . A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 1993; 366(6456):704-707. (Immunogen).
4. Yeager T, Stadler W, Belair C, Puthenveettil J, Olopade O, Reznikoff C. Increased p16 levels correlate with pRb alterations in human urothelial cells. Cancer Res. 1995; 55(3):493-497. (Clone-specific).
参考图片
Immunohistochemistry analysis of p16 expression on human breast tissue. A formalin-fixed, paraffin-embedded section from human breast tissue was stained with the Mouse Anti-Human p16 antibody (Cat. No. 550834). Cells expressing p16 were visualized using a three-step staining procedure with Polyclonal Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). P16 expression can be identified by the intense brown labeling of cell nuclei (magnification 20X).
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