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BD Pharmingen- APC Mouse Anti-Human CD45RA_BD Pharmingen

艾美捷科技 • 2025年08月19日 16:35:32 • 抗体 • 阅读 51 • 标签
产品信息
荧光素标记
APC
抗原名称
CD45RA
宿主
Mouse IgG2b, κ
简单描述
The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naïve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.
商品描述
HI100 The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naïve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.
同种型
Mouse IgG2b, κ
克隆号
克隆 HI100 (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD45RA
背景
别名
CD45R; PTPRC; LCA; Leukocyte common antigen
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(4) 1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997. 2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 3. Koristka S, Cartellieri M, Theil A, et al. Retargeting of human regulatory T cells by single-chain bispecific antibodies.. J Immunol. 2012; 188(3):1551-8. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 4. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
数据库链接
Entrez-Gene ID
5788

参考图片

Profile of peripheral blood lymphocytes analyzed by flow cytometry

Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Multicolor flow cytometric analysis of CD45RA expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD45RO antibody (Cat. No. 555493/561889), and with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745; Left Plot) or APC Mouse Anti-Human CD45RA antibody (Cat. No. 550855/561884; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD45RA (or Ig Isotype control) versus CD45RO was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

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