BD Pharmingen- APC Mouse Anti-Human CD62P_BD Pharmingen
产品信息
荧光素标记
APC
抗原名称
CD62P (P-Selectin)
宿主
Mouse BALB/c IgG1, κ
免疫原
Purified Human Platelet Membrane Glycoproteins
简单描述
The AK-4 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or Granule membrane protein-140 (GMP-140). CD62P is expressed by megakaryocytes, activated platelets and activated endothelium. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin is thought to mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.
商品描述
AK-4
The AK-4 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or Granule membrane protein-140 (GMP-140). CD62P is expressed by megakaryocytes, activated platelets and activated endothelium. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin is thought to mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 AK-4 (also known as AK4 or AK 4) (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD62P (P-Selectin)
背景
别名
GMP-140; GMP140; LECAM3; P-selectin; PADGEM; PSEL; SELP
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Johnson-Tidey RR, McGregor JL, Taylor PR, Poston RN. Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 1994; 144(5):952-961. (Biology).
2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
3. Lasky LA. Selectins: interpreters of cell-specific carbohydrate information during inflammation. Science. 1992; 258(5084):964-969. (Biology).
4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
参考图片
Flow cytometric analysis of CD62P expression on human platelets. Human platelets were activated with human thrombin (Sigma T-8885; 20 U/ml final concentration). The activated platelets were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Anti-Human CD62P antibody (Cat. No. 550888/561920; solid line histogram). The fluorescence histogram showing CD62P expression (or Ig Isotype control staining) was derived from events with the forward and side light-scatter characteristics of activated platelets.
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