Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.

(RUO)

Functional assay (Tested During Development)

BrdU Buffer

Aqueous buffered solution filtered through a 0.22 µm filter

Development References(7) 1. Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci U S A. 1983; 80(18):5573-5577. (Methodology). 2. Holm M, Thomsen M, Hoyer M, Hokland P. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 1998; 32(1):28-36. (Methodology). 3. Rocha B, Penit C, Baron C, Vasseur F, Dautigny N, Freitas AA. Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes. Eur J Immunol. 1990; 20(8):1697-1708. (Methodology). 4. Thoman ML. Early steps in T cell development are affected by aging. Cell Immunol. 1997; 178(2):117-123. (Methodology). 5. Tough DF, Sprent J. Turnover of naive- and memory-phenotype T cells. J Exp Med. 1994; 179(4):1127-1135. (Methodology). 6. deFazio A, Leary JA, Hedley DW, Tattersall MH. Immunohistochemical detection of proliferating cells in vivo. J Histochem Cytochem. 1987; 35(5):571-577. (Methodology). 7. von Boehmer H, Hafen K. The life span of naive alpha/beta T cells in secondary lymphoid organs. J Exp Med. 1993; 177(4):891-896. (Methodology).