This standard curve is for demonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (500 pg/mL), then adding 100 µL of the 500 pg/mL standard to both that well and the 250 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 250 pg/mL standard to the 125 pg/mL well. Continue these dilutions to the 7.8 pg/mL standard well, out of which the extra 100 µL should be discarded.