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BD Pharmingen- Purified Mouse Anti-Human CD197 -CCR7_BD Pharmingen

艾美捷科技 • 2025年08月19日 16:53:31 • 抗体 • 阅读 10 • 标签
产品信息
抗原名称
CD197
宿主
Mouse IgM, κ
免疫原
Human CCR7 transfected L1.2 mouse lymphoma cells
简单描述
The monoclonal antibody 2H4 reacts with the human CC chemokine receptor, CCR7/CD197. CCR7 (previously known as BLR-2, EBI-1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3•/Exodus 3/ELC/CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL214. It has been shown that CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. CCR7 mRNA was also detected in peripheral T- and B-lymphocytes, in BM and cord blood CD34-positive cells and mature dendritic cells. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17q12. The immunogen used to generate 2H4 hybridoma was human CCR7 transfected L1.2 mouse lymphoma cells. This antibody is not a neutralizing antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
2H4 The monoclonal antibody 2H4 reacts with the human CC chemokine receptor, CCR7/CD197. CCR7 (previously known as BLR-2, EBI-1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3•/Exodus 3/ELC/CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL214. It has been shown that CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. CCR7 mRNA was also detected in peripheral T- and B-lymphocytes, in BM and cord blood CD34-positive cells and mature dendritic cells. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17q12. The immunogen used to generate 2H4 hybridoma was human CCR7 transfected L1.2 mouse lymphoma cells. This antibody is not a neutralizing antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse IgM, κ
克隆号
克隆 2H4 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD197 (CCR7)
背景
别名
CCR7, BLR-2, EBI-1 and CMKBR7
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(9) 1. Birkenbach M, Josefsen K, Yalamanchili R, Lenoir G, Kieff E. Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors. Nature. 1993; 67(4):2209-2220. (Biology). 2. Burgstahler R, Kempkes B, Steube K, Lipp M. Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr virus nuclear antigen 2. Biochem Biophys Res Commun. 1995; 215(2):737-743. (Biology). 3. Kim CH, Pelus LM, White JR, Broxmeyer HE. Macrophage-inflammatory protein-3 beta/EBI1-ligand chemokine/CK beta-11, a CC chemokine, is a chemoattractant with a specificity for macrophage progenitors among myeloid progenitor cells. J Immunol. 1998; 161(5):2580-2585. (Biology). 4. Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature. 1999; 401(6754):708-712. (Biology). 5. Schweickart VL, Raport CJ, Godiska R, et al. Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics. 1994; 23(3):643-650. (Biology). 6. Yanagihara S, Komura E, Nagafune J, Watarai H, Yamaguchi Y. EBI1/CCR7 is a new member of dendritic cell chemokine receptor that is up-regulated upon maturation. J Immunol. 1998; 161(6):3096-3102. (Biology). 7. Yoshida R, Imai T, Hieshima K, et al. Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J Biol Chem. 1997; 272(21):13803-13809. (Biology). 8. Yoshida R, Nagira M, Imai T, et al. EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC. Int Immunol. 1998; 10(7):901-910. (Biology). 9. Yoshida R, Nagira M, Kitaura M, Imagawa N, Imai T, Yoshie O. Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. J Biol Chem. 1998; 273(12):7118-7122. (Biology).

参考图片

Detection of CCR7 expression on CD4 and CD8-positive human peripheral lymphocytes by purified anti-human CCR7 antibody 2H4 (Cat. No. 550937). Human PBMC were stained with 4.0 µg/10e6 of purified 2H4 using 3-step staining protocol outlined below and anti-human CD45RA-FITC (Cat. No. 555488). The data shown are derived from the CD4- positive (based on staining with anti-human CD4-APC, Cat. No. 555349, left panel) and CD8- positive (based on staining with anti-human CD8-APC, Cat. No. 555369, right panel) lymphocyte gated populations and displayed as bivariate dot plots. Cells were analyzed with a FACScan™ Flow Cytometer (BD Biosciences, San Jose, CA)

Detection of CCR7 expression on CD4 and CD8-positive human peripheral lymphocytes by purified anti-human CCR7 antibody 2H4 (Cat. No. 550937). Human PBMC were stained with 4.0 µg/10e6 of purified 2H4 using 3-step staining protocol outlined below and anti-human CD45RA-FITC (Cat. No. 555488). The data shown are derived from the CD4- positive (based on staining with anti-human CD4-APC, Cat. No. 555349, left panel) and CD8- positive (based on staining with anti-human CD8-APC, Cat. No. 555369, right panel) lymphocyte gated populations and displayed as bivariate dot plots. Cells were analyzed with a FACScan™ Flow Cytometer (BD Biosciences, San Jose, CA)

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