产品信息
抗原名称
Streptavidin
简单描述
Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules to stain target molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Horseradish Peroxidase (HRP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, Streptavidin HRP has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.
商品描述
Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules to stain target molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Horseradish Peroxidase (HRP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, Streptavidin HRP has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.
克隆号
(RUO)
产品详情
HRP
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide resulting in a visible colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with HRP and used in applications such as i) ELISA that uses a plate reader to measure absorbance of the colored product, or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
应用
实验应用
Immunohistochemistry (Routinely Tested)
目标/特异性
Streptavidin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
保存方式
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
参考图片
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