BD Pharmingen- PerCP-Cy-5-5 Mouse Anti-Mouse NK-1-1_BD Pharmingen
产品信息
荧光素标记
PerCP-Cy5.5
抗原名称
NK1.1
宿主
Mouse C3H x BALB/c IgG2a, κ
免疫原
Mouse NK-1+ Spleen and Bone Marrow Cells
简单描述
In the mouse, at least three members of the
Klrb
(
K
iller cell
l
ectin-like
r
eceptor, subfamily
b
; formerly
NKR-P1
) gene family have been identified (
Klrb1a/NKR-P1A, Klrb1b/NKR-P1B,
and
Klrb1c/NKR-P1C
); but in the human gene family, a single homologue has been designated
KLRB1, NKR-P1A, or CD161
. The KLRB1/NKR-P1 family of proteins are type-II-transmembrane C-type lectin receptors. KLRB1C/NKR-P1C activates NK-cell cytotoxicity, while KLRB1B/NKR-P1B functions as an inhibitory receptor. KLRB1B/NKR-P1B protein has intracellular
I
mmunoreceptor
T
yrosine-based
I
nhibitory
M
otif (ITIM), while KLRB1C/NKR-P1C lacks ITIM and activates via association with Fc Receptor γ chain. Strikingly, KLRB1B/NKR-P1B and KLRB1C/NKR-P1C share 96% amino acid sequence identity in their extracellular C-type lectin domains. The PK136 antibody reacts with the NK-1.1 surface antigen (CD161c) encoded by the
Klrb1c/NKR-P1C
gene expressed on natural killer (NK) cells in selected strains of mice (eg, C57BL, FVB/N, NZB, but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, 129) and the CD161b antigen encoded by the
Klrb1b/NKR-P1B
gene expressed only on Swiss NIH and SJL mice, but not on C57BL/6. Expression of KLRB1C/NKR-P1C protein is correlated with the ability to lyse tumor cells in vitro and to mediate rejection of bone marrow allografts. The NK-1.1 marker is useful in defining NK cells; however, the antigen is also expressed on a rare, specialized population of T lymphocytes (NK-T cells) and some cultured monocytes. Plate-bound PK136 mAb, in combination with low concentrations of IL-2, induces proliferation of a subset of NK cells.
商品描述
PK136
In the mouse, at least three members of the
Klrb
(
K
iller cell
l
ectin-like
r
eceptor, subfamily
b
; formerly
NKR-P1
) gene family have been identified (
Klrb1a/NKR-P1A, Klrb1b/NKR-P1B,
and
Klrb1c/NKR-P1C
); but in the human gene family, a single homologue has been designated
KLRB1, NKR-P1A, or CD161
. The KLRB1/NKR-P1 family of proteins are type-II-transmembrane C-type lectin receptors. KLRB1C/NKR-P1C activates NK-cell cytotoxicity, while KLRB1B/NKR-P1B functions as an inhibitory receptor. KLRB1B/NKR-P1B protein has intracellular
I
mmunoreceptor
T
yrosine-based
I
nhibitory
M
otif (ITIM), while KLRB1C/NKR-P1C lacks ITIM and activates via association with Fc Receptor γ chain. Strikingly, KLRB1B/NKR-P1B and KLRB1C/NKR-P1C share 96% amino acid sequence identity in their extracellular C-type lectin domains. The PK136 antibody reacts with the NK-1.1 surface antigen (CD161c) encoded by the
Klrb1c/NKR-P1C
gene expressed on natural killer (NK) cells in selected strains of mice (eg, C57BL, FVB/N, NZB, but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, 129) and the CD161b antigen encoded by the
Klrb1b/NKR-P1B
gene expressed only on Swiss NIH and SJL mice, but not on C57BL/6. Expression of KLRB1C/NKR-P1C protein is correlated with the ability to lyse tumor cells in vitro and to mediate rejection of bone marrow allografts. The NK-1.1 marker is useful in defining NK cells; however, the antigen is also expressed on a rare, specialized population of T lymphocytes (NK-T cells) and some cultured monocytes. Plate-bound PK136 mAb, in combination with low concentrations of IL-2, induces proliferation of a subset of NK cells.
同种型
Mouse C3H x BALB/c IgG2a, κ
克隆号
克隆 PK136 (RUO)
浓度
0.2 mg/ml
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD161b,c (NK-1.1)
背景
别名
Klrb1b, CD161b, Nkrp1b; Klrb1c, CD161c, NK1.1, Nkrp1c
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Carlyle JR, Martin A, Mehra A, Attisano L, Tsui FW, Zuniga-Pflucker JC. Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function. J Immunol. 1999; 162(10):5917-5923. (Clone-specific: Immunoprecipitation).
2. Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J. Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets. Cytometry. 1996; 23(3):205-217. (Biology).
3. Koo GC, Peppard JR. Establishment of monoclonal anti-Nk-1.1 antibody. Hybridoma. 1984; 3(3):301-303. (Immunogen: Cytotoxicity, Flow cytometry).
4. Kung SK, Su RC, Shannon J, Miller RG. The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells. J Immunol. 1999; 162(10):5876-5887. (Clone-specific: Blocking).
5. Reichlin A, Yokoyama WM. Natural killer cell proliferation induced by anti-NK1.1 and IL-2. Immunol Cell Biol. 1998; 76(2):143-152. (Clone-specific: Induction).
参考图片
Two-color analysis of NK-1.1 expression on lymphokine-activated killer (LAK) cells. LAK cells from C57BL/6 mice were stained with FITC-conjugated anti-mouse CD244.2 mAb 2B4 (Cat. No. 553305) and either PerCP-Cy5.5-conjugated mouse IgG2a κ isotype control mAb G155-178 (Cat. No. 551115, left panel) or PerCP-Cy5.5-conjugated mAb PK136 (right panel). Viable blasts were gated according to their light-scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Two-color analysis of NK-1.1 expression on lymphokine-activated killer (LAK) cells. LAK cells from C57BL/6 mice were stained with FITC-conjugated anti-mouse CD244.2 mAb 2B4 (Cat. No. 553305) and either PerCP-Cy5.5-conjugated mouse IgG2a κ isotype control mAb G155-178 (Cat. No. 551115, left panel) or PerCP-Cy5.5-conjugated mAb PK136 (right panel). Viable blasts were gated according to their light-scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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