CD73

Mouse BALB/c IgG1, κ

Ecto-5'-nucleotidase purified from rat renal membranes

The 5F/B9 monoclonal antibody specifically recognizes CD73 or Ecto-5'-nucleotidase (5'-NT), a 68-kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities.  5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues.  Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart.  The expression of CD73 on mouse leukocytes has been well characterized.  In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes,  NK cells,  and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes.  The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT.  Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18.

The 5F/B9 monoclonal antibody specifically recognizes CD73 or Ecto-5"-nucleotidase (5"-NT), a 68-kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities.  5"-NT catalyzes the dephosphorylation of extracellular nucleoside 5" monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues.  Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart.  The expression of CD73 on mouse leukocytes has been well characterized.  In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes,  NK cells,  and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes.  The 5F/B9 mAb does not inhibit the enzymatic activity of 5"-NT.  Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18.

Mouse BALB/c IgG1, κ

5F/B9

0.5 mg/ml

Flow cytometry (Routinely Tested) Immunohistochemistry-zinc-fixed, Immunohistochemistry-frozen, Immunofluorescence (Tested During Development) (Co)-stimulation, Immunoprecipitation (Reported) Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)

Rat (QC Testing)

Ecto-5'-nucleotidase; Nt5e; Nt5; 5'-NT

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(6) 1. Carvalho GS, Luzio JP, Siddle K, Coombs RR. Detection of ecto-5'-nucleotidase on rat B and T lymphocyte subpopulations using a monoclonal antibody in rosetting reactions. J Immunol Methods. 1987; 102(1):119-126. (Biology). 2. Gandhi R, Le Hir M, Kaissling B. Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody. Histochem J. 1990; 95(2):165-174. (Immunogen: Immunohistochemistry, Immunoprecipitation). 3. Mlodzik K, Loffing J, Le Hir M, Kaissling B. Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart. Histochem J. 1995; 103(3):227-236. (Biology). 4. Yamashita Y, Hooker SW, Jiang H, et al. CD73 expression and fyn-dependent signaling on murine lymphocytes. Eur J Immunol. 1998; 28(10):2981-2990. (Biology). 5. Zenker W, Rinne B, Bankoul S, Le Hir M, Kaissling B. 5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study. Histochem J. 1992; 98(2):135-139. (Biology). 6. Zimmermann H. 5'-Nucleotidase: molecular structure and functional aspects. Biochem J. 1992; 285((Pt 2)):345-365. (Biology).