BD Pharmingen- PE-Cy-5 Mouse Anti-Human CD137_BD Pharmingen
产品信息
荧光素标记
PE-Cy5
抗原名称
CD137
宿主
Mouse BALB/c IgG1, κ
免疫原
Recombinant Human 4-1BB Fusion Protein
简单描述
The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). CD137 is a type I transmembrane glycoprotein that belongs to the TNF/NGF receptor family. It is encoded by
TNFRSF9
(tumor necrosis factor receptor superfamily, member 9). CD137 is expressed on activated T cells, B cells, monocytes, and follicular dendritic cells. CD137 plays roles in the costimulation, differentiation and survival of T cells and B cells.
商品描述
4B4-1
The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). CD137 is a type I transmembrane glycoprotein that belongs to the TNF/NGF receptor family. It is encoded by
TNFRSF9
(tumor necrosis factor receptor superfamily, member 9). CD137 is expressed on activated T cells, B cells, monocytes, and follicular dendritic cells. CD137 plays roles in the costimulation, differentiation and survival of T cells and B cells.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 4B4-1 (RUO)
产品详情
PE-Cy5
PE-Cy5 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496 nm and 566 nm and an acceptor dye, Cy™5, with an emission maximum (Em Max) at 670-nm. PE designed to be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 670-nm (e.g., a 670/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627-640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-Cy5
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
670 nm
组成成分
免疫球蛋白G型抗体
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD137 (4-1BB)
背景
别名
4-1BB, ILA, TNFRSF9
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Garni-Wagner BA, Lee ZH, Kim YJ, Wilde C, Kang CY, Kwon BS. 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans. Cell Immunol. 1996; 169(1):91-98. (Biology).
2. Hurtado JC, Kim SH, Pollok KE, Lee ZH, Kwon BS. Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28. J Immunol. 1995; 155(7):3360-3367. (Biology).
3. Hurtado JC, Kim YJ, Kwon BS. AAI Meeting, Abstract #841. San Francisco: 1997.
4. Kim YJ, Kim SH, Kwon BS. AAI Meeting, Abstract #838. San Francisco: 1997.
5. Zhou Z, Kim S, Hurtado J, et al. Characterization of human homologue of 4-1BB and its ligand. Immunol Lett. 1995; 45(1-2):67-73. (Biology).
数据库链接
Entrez-Gene ID
3604
参考图片
Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A-stimulated (3 days) peripheral blood mononuclear cells were stained with either PE-Cy5 Mouse IgG1, k Isotype Control (Cat. No. 555750; dashed line histogram) or PE-Cy5 Mouse Anti-Human CD137 antibody (Cat. No. 551137; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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