1. 首页
  2. 抗体
  3. 正文

Hu Cyclin E W-Cont Pure HE12 50ug_BD Pharmingen

艾美捷科技 • 2025年08月20日 09:28:59 • 抗体 • 阅读 8 • 标签
产品信息
荧光素标记
Unconjugated
抗原名称
Cyclin-E
商品描述
Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle.  These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27 [Kip1].  Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase where it plays an important role in the regulation of the G1/S restriction checkpoint in the cell cycle. Abberant expression of cyclin E has been reported to be associated with the oncogenic transformation of cells.  This antibody has been reported not to cross-react with mouse cyclin E.
克隆号
HE12
应用
实验应用
Western blot (Routinely Tested) Bioimaging (Tested During Development) Flow cytometry (Reported)
反应种属
Human (QC Testing)

参考图片

Left: Western blot analysis of Cyclin E. Lysate from HeLa cells was probed with the anti-cyclin E antibody at concentrations of 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/mL (lane 3). Cyclin E is identified as a band of 50 kDa. Right: Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-Cyclin E antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager system using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure).

文档下载: W 导出为Hu Cyclin E W-Cont Pure HE12 50ug_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8892.html

赞 (8)
打赏 微信扫一扫
« 上一篇 2025年08月20日 09:27:48
下一篇 » 2025年08月20日 09:30:38

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();