BD Pharmingen- Purified Rat Anti-Mouse IL-10_BD Pharmingen
产品信息
抗原名称
IL-10
宿主
Rat IgG1, κ
免疫原
Mouse IL-10
简单描述
The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.
This antibody is routinely tested by ELISA capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
JES5-2A5
The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.
This antibody is routinely tested by ELISA capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG1, κ
克隆号
克隆 JES5-2A5 (RUO)
浓度
1.0 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested), Western blot (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
IL-10
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization).
3. Mo XY, Sarawar SR, Doherty PC. Induction of cytokines in mice with parainfluenza pneumonia. J Virol. 1995; 69(2):1288-1291. (Clone-specific: ELISA).
4. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA).
5. Sarawar SR, Sangster M, Coffman RL, Doherty PC. Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype. J Immunol. 1994; 153(3):1246-1253. (Clone-specific: ELISA).
参考图片
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