BD Pharmingen- PerCP-Cy-5-5 Mouse Anti-Human CD38_BD Pharmingen
产品信息
荧光素标记
PerCP-Cy5.5
抗原名称
CD38
宿主
Mouse IgG1, κ
简单描述
The HIT2 monoclonal antibody specifically binds to CD38. The CD38 antigen is also known as T10, ADP-ribosyl cyclase 1, and cyclic ADP ribose hydrolase 1. CD38 is a 45 kDa type II single-chain transmembrane glycoprotein present on thymocytes, activated T cells and terminally differentiated B cells (plasma cells). CD38 is expressed by other cells including monocytes, macrophages, dendritic cells, NK cells, myeloid and erythroid precursors and some epithelial cells. The CD38 antigen acts as an ectoenzyme that catalyzes the synthesis and hydrolysis of a Ca++ mobilizing agent, cyclic ADP-ribose. This intracellular calcium plays an important role in cell signaling pathways leading to cellular growth, apoptosis, and differentiation. CD38 binds to CD31 and thus plays a role in lymphocyte adhesion to endothelial cells.
商品描述
HIT2
The HIT2 monoclonal antibody specifically binds to CD38. The CD38 antigen is also known as T10, ADP-ribosyl cyclase 1, and cyclic ADP ribose hydrolase 1. CD38 is a 45 kDa type II single-chain transmembrane glycoprotein present on thymocytes, activated T cells and terminally differentiated B cells (plasma cells). CD38 is expressed by other cells including monocytes, macrophages, dendritic cells, NK cells, myeloid and erythroid precursors and some epithelial cells. The CD38 antigen acts as an ectoenzyme that catalyzes the synthesis and hydrolysis of a Ca++ mobilizing agent, cyclic ADP-ribose. This intracellular calcium plays an important role in cell signaling pathways leading to cellular growth, apoptosis, and differentiation. CD38 binds to CD31 and thus plays a role in lymphocyte adhesion to endothelial cells.
同种型
Mouse IgG1, κ
克隆号
克隆 HIT2 (RUO)
浓度
0.2 mg/ml
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD38
背景
别名
T10; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; gp45
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(11)
1. Deaglio S, Morra M, Mallone R, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol. 1998; 160(1):395-402. (Biology).
2. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD38. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:86.
3. Hernandez-Lopez C, Varas A, Sacedon R, et al. Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood. 2002; 99(2):546-554. (Clone-specific: Flow cytometry).
4. Jackson DG, Bell JI. Isolation of a cDNA encoding the human CD38 (T10) molecule, a cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation. J Immunol. 1990; 144(7):2811-2815. (Clone-specific: Cell separation, Immunoprecipitation).
5. Jourdan M, Caraux A, Caron G, et al. Characterization of a transitional preplasmablast population in the process of human B cell to plasma cell differentiation. J Immunol. 2011; 187(8):3931-3941. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting).
6. Lanier LL, Le AM, Ding AH, Evans EL. Analysis of the Workshop T-cell monoclonal antibodies by 'Indirect two-colour immunofluorescense' and multiparameter flow cytometry. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:62-68.
7. McMichael AJ, Gotch FM. T-cell antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:31-62.
8. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
9. Roy MP, Kim CH, Butcher EC. Cytokine control of memory B cell homing machinery. J Immunol. 2002; 169(4):1676-1682. (Clone-specific: Flow cytometry).
10. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
11. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
参考图片
Profile of resting peripheral blood lymphocytes analyzed by flow cytometry
Profile of resting peripheral blood lymphocytes analyzed by flow cytometry
Multivariate flow cytometric analysis of CD38 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG1 κ Isotype Control (Cat. No. 550795; Left Plot) or PerCP-Cy5.5 Mouse Anti-Human CD38 antibody (Cat. No. 551400/561106; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing CD38 expression (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD™ LSR II Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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