产品信息
荧光素标记
PE
抗原名称
IL-6
宿主
Rat IgG2a, κ
免疫原
Recombinant human IL-6
简单描述
IL-6, a 26 kDa protein, is a multifunctional cytokine secreted by both lymphoid and non-lymphoid cells. It regulates B and T cell function and hematopoiesis. The MQ2-6A3 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate the MQ2-6A3 hybridoma was recombinant human IL-6. This is a neutralizing antibody. Clone MQ2-6A3 cross-reacts with an intracellular component of peripheral blood LPS-stimulated permeabilized monocytes of rhesus and cynomolgus macaques. The reactivity pattern observed on non-human primates is similar, although weaker, than that seen on normal human monocytes.
商品描述
MQ2-6A3
IL-6, a 26 kDa protein, is a multifunctional cytokine secreted by both lymphoid and non-lymphoid cells. It regulates B and T cell function and hematopoiesis. The MQ2-6A3 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate the MQ2-6A3 hybridoma was recombinant human IL-6. This is a neutralizing antibody. Clone MQ2-6A3 cross-reacts with an intracellular component of peripheral blood LPS-stimulated permeabilized monocytes of rhesus and cynomolgus macaques. The reactivity pattern observed on non-human primates is similar, although weaker, than that seen on normal human monocytes.
同种型
Rat IgG2a, κ
克隆号
克隆 MQ2-6A3 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Rhesus, Cynomolgus (QC Testing)
目标/特异性
IL-6
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Villinger F, Brar SS, Mayne A, Chikkala N, Ansari AA. Comparative sequence analysis of cytokine genes from human and nonhuman primates. J Immunol. 1995; 155(8):3946-3954. (Biology).
2. Villinger F, Hunt D, Mayne A, Vuchetich M, Findley H, Ansari AA. Qualitative and quantitative studies of cytokines synthesized and secreted by non-human primate peripheral blood mononuclear cells. Cytokine. 1993; 5(5):469-479. (Biology).
参考图片
Profile of LPS-stimulated (24 hrs) peripheral blood monocytes of rhesus macaque monkey (Macaca mulatta) analyzed by flow cytometry
Profile of LPS-stimulated (24 hrs) peripheral blood monocytes of rhesus macaque monkey (Macaca mulatta) analyzed by flow cytometry
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