产品信息
荧光素标记
PE
抗原名称
CD184
宿主
Rat IgG2a, κ
免疫原
Human CXCR4 fusion protein
简单描述
The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG2a, κ
克隆号
1D9
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
背景
别名
CXCR4, Fusin
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(11)
1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology).
2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology).
3. Federsppiel B, Melhado IG, Duncan AM, et al. Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated from human spleen. Genomics. 1993; 16(3):707-712. (Biology).
4. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology).
5. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen).
6. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology).
7. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997; 385(6617):645-649. (Biology).
8. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology).
9. Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology).
10. Nomura H, Nielsen BW, Matsushima K. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. Int Immunol. 1993; 5(10):1239-1249. (Biology).
11. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology).
参考图片
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