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BD Pharmingen- Biotin Rat Anti-Mouse Pre-B Cell Receptor_BD Pharmingen

艾美捷科技 • 2025年08月20日 11:17:03 • 抗体 • 阅读 8 • 标签
产品信息
抗原名称
Pre-B Cell Receptor
宿主
Rat LEW, also known as Lewis IgG2a, κ
免疫原
Purified soluble complex of recombinant µ IgH, λ5, and VpreB
简单描述
The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked Lambda 5 (λ5) and VpreB proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The SL156 antibody reacts with a conformational epitope of the pre-BCR in transfected X63-Ag8.653 cells but not with surrogate light chain, λ5, or VpreB in the absence of IgH. It detects pre-BCR on pre-B cell lines, but not on pro-B cell lines or IgM-positive splenocytes. It also detects pre-BCR on the surface of pre-B cells, but not on Ig light chain (IgL)-positive B lymphocytes, from the bone marrow of normal mice. It has been noted that the cell-surface expression of pre-BCR is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C. After immunization, cell-surface pre-BCR is detected on a subset of splenic IgL+ germinal-center B lymphocytes.
商品描述
SL156 The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked Lambda 5 (λ5) and VpreB proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The SL156 antibody reacts with a conformational epitope of the pre-BCR in transfected X63-Ag8.653 cells but not with surrogate light chain, λ5, or VpreB in the absence of IgH. It detects pre-BCR on pre-B cell lines, but not on pro-B cell lines or IgM-positive splenocytes. It also detects pre-BCR on the surface of pre-B cells, but not on Ig light chain (IgL)-positive B lymphocytes, from the bone marrow of normal mice. It has been noted that the cell-surface expression of pre-BCR is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C. After immunization, cell-surface pre-BCR is detected on a subset of splenic IgL+ germinal-center B lymphocytes.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 SL156 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
Pre-B Cell Receptor
背景
别名
Pre-BCR
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Karasuyama H, Rolink A, Melchers F. A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines. J Exp Med. 1993; 178(2):469-478. (Immunogen). 2. Martensson IL, Ceredig R. Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development. Immunology. 2000; 101(4):435-441. (Biology). 3. Meffre E, Papavasiliou F, Cohen P, et al. Antigen receptor engagement turns off the V(D)J recombination machinery in human tonsil B cells. J Exp Med. 1998; 188(4):765-772. (Biology). 4. Melchers F, ten Boekel E, Seidl T, et al. Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells. Immunol Rev. 2000; 175:33-46. (Biology). 5. Shimizu T, Mundt C, Licence S, Melchers F, Martensson IL. VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus. J Immunol. 2002; 168(12):6286-6293. (Biology). 6. Winkler TH, Rolink A, Melchers F, Karasuyama H. Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface. Eur J Immunol. 1995; 25(2):446-450. (Immunogen).

参考图片

Two-color analysis of Pre-B cell receptor (Pre-BCR) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM[b] mAb AF6-78 (Cat. No. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37°C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553928, left panel) or biotinylated mAb SL156 (right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by Streptavidin-APC (Cat. No. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb (Cat. No. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Two-color analysis of Pre-B cell receptor (Pre-BCR) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM[b] mAb AF6-78 (Cat. No. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37°C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553928, left panel) or biotinylated mAb SL156 (Cat. No. 551863, right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by Streptavidin-APC (Cat. No. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb (Cat. No. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

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